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Comparison of the binding of chicken alpha-macroglobulin and ovomacroglobulin to the mammalian alpha 2-macroglobulin receptor.

Publication ,  Journal Article
Feldman, SR; Pizzo, SV
Published in: Arch Biochem Biophys
November 15, 1984

Chicken alpha-macroglobulin (alpha M) and ovomacroglobulin were purified by Ni+2 chelate chromatography. These proteins had similar subunit structure as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Chicken alpha M bound 1.0 mol and ovomacroglobulin bound 0.8 mol 125I-trypsin per mol inhibitor, respectively. Ovomacroglobulin cleared rapidly from the circulation of mice, and the clearance was inhibited by asialoorosomucoid, but native chicken alpha M cleared slowly (t 1/2 greater than 1 h). After reaction with trypsin, this alpha-macroglobulin cleared rapidly (t 1/2 = 3 min), and this clearance was inhibited by a 1000-fold molar excess of human alpha 2M-methylamine. Ovomacroglobulin-trypsin did not inhibit the binding of 0.2 nM 125I-labeled human alpha 2M-methylamine to mouse peritoneal macrophages in vitro, but chicken alpha M reacted with trypsin inhibited the binding by 50% at 1.9 nM. A kappa I of 1.1 nM was calculated for the binding of chicken alpha M-trypsin to the mammalian alpha-macroglobulin receptor. This affinity is comparable to that obtained with human and bovine alpha 2M.

Duke Scholars

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

November 15, 1984

Volume

235

Issue

1

Start / End Page

267 / 275

Location

United States

Related Subject Headings

  • alpha-Macroglobulins
  • Trypsin
  • Receptors, Immunologic
  • Macroglobulins
  • Low Density Lipoprotein Receptor-Related Protein-1
  • Electrophoresis, Polyacrylamide Gel
  • Chickens
  • Biochemistry & Molecular Biology
  • Animals
  • Amino Acids
 

Citation

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MLA
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Feldman, S. R., & Pizzo, S. V. (1984). Comparison of the binding of chicken alpha-macroglobulin and ovomacroglobulin to the mammalian alpha 2-macroglobulin receptor. Arch Biochem Biophys, 235(1), 267–275. https://doi.org/10.1016/0003-9861(84)90275-3
Feldman, S. R., and S. V. Pizzo. “Comparison of the binding of chicken alpha-macroglobulin and ovomacroglobulin to the mammalian alpha 2-macroglobulin receptor.Arch Biochem Biophys 235, no. 1 (November 15, 1984): 267–75. https://doi.org/10.1016/0003-9861(84)90275-3.
Feldman, S. R., and S. V. Pizzo. “Comparison of the binding of chicken alpha-macroglobulin and ovomacroglobulin to the mammalian alpha 2-macroglobulin receptor.Arch Biochem Biophys, vol. 235, no. 1, Nov. 1984, pp. 267–75. Pubmed, doi:10.1016/0003-9861(84)90275-3.
Journal cover image

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

November 15, 1984

Volume

235

Issue

1

Start / End Page

267 / 275

Location

United States

Related Subject Headings

  • alpha-Macroglobulins
  • Trypsin
  • Receptors, Immunologic
  • Macroglobulins
  • Low Density Lipoprotein Receptor-Related Protein-1
  • Electrophoresis, Polyacrylamide Gel
  • Chickens
  • Biochemistry & Molecular Biology
  • Animals
  • Amino Acids