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Monoclonal antibody and F(ab')2 fragment delivery to tumor in patients with glioma: comparison of intracarotid and intravenous administration.

Publication ,  Journal Article
Zalutsky, MR; Moseley, RP; Benjamin, JC; Colapinto, EV; Fuller, GN; Coakham, HP; Bigner, DD
Published in: Cancer Res
July 1, 1990

Non-i.v. delivery of radiolabeled monoclonal antibodies (MAbs) has been shown to increase tumor uptake and decrease dose to normal tissues. In this study, we have examined the potential advantage of intracarotid (i.c.) versus i.v. administration for the delivery of an intact MAb and a F(ab')2 fragment to tumor in patients with gliomas. Three patients received 10-50 mg of 81C6 IgG2b, a MAb reactive with the glioma-associated extracellular matrix antigen tenascin, and three received 5-20 mg of the F(ab')2 fragment of Mel-14, which is reactive with gliomas and melanomas. Paired-injection protocols, in which one-half of the MAb was labeled with 131I and administered by i.c. injection, and one-half was labeled with 125I and simultaneously administered by i.v. injection, were used. For both 81C6 IgG2b and Mel-14 (Fab')2, no differences in blood clearance half-times or urinary excretion rates of radioiodine were observed between i.c.- and i.v.-administered activity. Analysis of biopsy samples revealed i.c.:i.v. uptake ratios of 1.02 +/- 0.04, 0.95 +/- 0.03, and 1.03 +/- 0.05 for the accumulation of 81C6 IgG2b in temporalis muscle, normal brain, and glioma, respectively. Similarly, the i.c.:i.v. uptake ratios for Mel-14 F(ab')2 in these tissues were 0.98 +/- 0.04 (SD), 1.00 +/- 0.05, and 1.04 +/- 0.05. When the differences in percentage of injected dose/g uptake after i.c. and i.v. administration were compared, no statistically significant advantage for i.c. delivery was seen (P = 0.22-0.61). These data indicate that i.c. administration of MAb 81C6 IgG2b and Mel-14 F(ab')2 fragments offers no delivery advantage to offset the small but finite risk involved in cannulation and injection of the internal carotid artery.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

July 1, 1990

Volume

50

Issue

13

Start / End Page

4105 / 4110

Location

United States

Related Subject Headings

  • Radionuclide Imaging
  • Oncology & Carcinogenesis
  • Muscles
  • Iodine Radioisotopes
  • Injections, Intravenous
  • Injections, Intra-Arterial
  • Immunoglobulin Fab Fragments
  • Humans
  • Glioblastoma
  • Carotid Arteries
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zalutsky, M. R., Moseley, R. P., Benjamin, J. C., Colapinto, E. V., Fuller, G. N., Coakham, H. P., & Bigner, D. D. (1990). Monoclonal antibody and F(ab')2 fragment delivery to tumor in patients with glioma: comparison of intracarotid and intravenous administration. Cancer Res, 50(13), 4105–4110.
Zalutsky, M. R., R. P. Moseley, J. C. Benjamin, E. V. Colapinto, G. N. Fuller, H. P. Coakham, and D. D. Bigner. “Monoclonal antibody and F(ab')2 fragment delivery to tumor in patients with glioma: comparison of intracarotid and intravenous administration.Cancer Res 50, no. 13 (July 1, 1990): 4105–10.
Zalutsky MR, Moseley RP, Benjamin JC, Colapinto EV, Fuller GN, Coakham HP, et al. Monoclonal antibody and F(ab')2 fragment delivery to tumor in patients with glioma: comparison of intracarotid and intravenous administration. Cancer Res. 1990 Jul 1;50(13):4105–10.
Zalutsky MR, Moseley RP, Benjamin JC, Colapinto EV, Fuller GN, Coakham HP, Bigner DD. Monoclonal antibody and F(ab')2 fragment delivery to tumor in patients with glioma: comparison of intracarotid and intravenous administration. Cancer Res. 1990 Jul 1;50(13):4105–4110.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

July 1, 1990

Volume

50

Issue

13

Start / End Page

4105 / 4110

Location

United States

Related Subject Headings

  • Radionuclide Imaging
  • Oncology & Carcinogenesis
  • Muscles
  • Iodine Radioisotopes
  • Injections, Intravenous
  • Injections, Intra-Arterial
  • Immunoglobulin Fab Fragments
  • Humans
  • Glioblastoma
  • Carotid Arteries