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Requirement of the prolyl isomerase Pin1 for the replication checkpoint.

Publication ,  Journal Article
Winkler, KE; Swenson, KI; Kornbluth, S; Means, AR
Published in: Science
March 3, 2000

The peptidyl-prolyl isomerase Pin1 has been implicated in regulating cell cycle progression. Pin1 was found to be required for the DNA replication checkpoint in Xenopus laevis. Egg extracts depleted of Pin1 inappropriately transited from the G2 to the M phase of the cell cycle in the presence of the DNA replication inhibitor aphidicolin. This defect in replication checkpoint function was reversed after the addition of recombinant wild-type Pin1, but not an isomerase-inactive mutant, to the depleted extract. Premature mitotic entry in the absence of Pin1 was accompanied by hyperphosphorylation of Cdc25, activation of Cdc2/cyclin B, and generation of epitopes recognized by the mitotic phosphoprotein antibody, MPM-2. Therefore, Pin1 appears to be required for the checkpoint delaying the onset of mitosis in response to incomplete replication.

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Published In

Science

DOI

ISSN

0036-8075

Publication Date

March 3, 2000

Volume

287

Issue

5458

Start / End Page

1644 / 1647

Location

United States

Related Subject Headings

  • cdc25 Phosphatases
  • Xenopus laevis
  • Xenopus Proteins
  • Recombinant Proteins
  • Protein-Tyrosine Kinases
  • Protein Serine-Threonine Kinases
  • Protein Kinases
  • Point Mutation
  • Peptidylprolyl Isomerase
  • Oocytes
 

Citation

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Winkler, K. E., Swenson, K. I., Kornbluth, S., & Means, A. R. (2000). Requirement of the prolyl isomerase Pin1 for the replication checkpoint. Science, 287(5458), 1644–1647. https://doi.org/10.1126/science.287.5458.1644
Winkler, K. E., K. I. Swenson, S. Kornbluth, and A. R. Means. “Requirement of the prolyl isomerase Pin1 for the replication checkpoint.Science 287, no. 5458 (March 3, 2000): 1644–47. https://doi.org/10.1126/science.287.5458.1644.
Winkler KE, Swenson KI, Kornbluth S, Means AR. Requirement of the prolyl isomerase Pin1 for the replication checkpoint. Science. 2000 Mar 3;287(5458):1644–7.
Winkler, K. E., et al. “Requirement of the prolyl isomerase Pin1 for the replication checkpoint.Science, vol. 287, no. 5458, Mar. 2000, pp. 1644–47. Pubmed, doi:10.1126/science.287.5458.1644.
Winkler KE, Swenson KI, Kornbluth S, Means AR. Requirement of the prolyl isomerase Pin1 for the replication checkpoint. Science. 2000 Mar 3;287(5458):1644–1647.
Journal cover image

Published In

Science

DOI

ISSN

0036-8075

Publication Date

March 3, 2000

Volume

287

Issue

5458

Start / End Page

1644 / 1647

Location

United States

Related Subject Headings

  • cdc25 Phosphatases
  • Xenopus laevis
  • Xenopus Proteins
  • Recombinant Proteins
  • Protein-Tyrosine Kinases
  • Protein Serine-Threonine Kinases
  • Protein Kinases
  • Point Mutation
  • Peptidylprolyl Isomerase
  • Oocytes