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Swelling and mechanical behaviors of chemically cross-linked hydrogels of elastin-like polypeptides.

Publication ,  Journal Article
Trabbic-Carlson, K; Setton, LA; Chilkoti, A
Published in: Biomacromolecules
May 2003

Genetically engineered elastin-like polypeptides consisting of Val-Pro-Gly-X-Gly repeats, where X was chosen to be Lys every 7 or 17 pentapeptides (otherwise X was Val), were synthesized and expressed in E. coli, purified, and chemically cross-linked using tris-succinimidyl aminotriacetate to produce hydrogels. Swelling experiments indicate hydrogel mass decreases by 80-90% gradually over an approximate 50 degrees C temperature range. Gels ranged in stiffness from 0.24 to 3.7 kPa at 7 degrees C and from 1.6 to 15 kPa at 37 degrees C depending on protein concentration, lysine content, and molecular weight. Changes in gel stiffness and loss angle with cross-linking formulation suggest a low-temperature gel structure that is nearly completely elastic, where force is transmitted almost exclusively through fully extended polypeptide chains and chemical cross-links, and a high-temperature gel structure, where ELP chains are contracted and force is transmitted through chemical cross-links as well as frictional contact between polypeptide chains.

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Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

May 2003

Volume

4

Issue

3

Start / End Page

572 / 580

Related Subject Headings

  • Thermodynamics
  • Temperature
  • Stress, Mechanical
  • Polymers
  • Peptides
  • Molecular Weight
  • Hydrogels
  • Cross-Linking Reagents
  • 40 Engineering
  • 34 Chemical sciences
 

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Trabbic-Carlson, K., Setton, L. A., & Chilkoti, A. (2003). Swelling and mechanical behaviors of chemically cross-linked hydrogels of elastin-like polypeptides. Biomacromolecules, 4(3), 572–580. https://doi.org/10.1021/bm025671z
Trabbic-Carlson, Kimberly, Lori A. Setton, and Ashutosh Chilkoti. “Swelling and mechanical behaviors of chemically cross-linked hydrogels of elastin-like polypeptides.Biomacromolecules 4, no. 3 (May 2003): 572–80. https://doi.org/10.1021/bm025671z.
Trabbic-Carlson K, Setton LA, Chilkoti A. Swelling and mechanical behaviors of chemically cross-linked hydrogels of elastin-like polypeptides. Biomacromolecules. 2003 May;4(3):572–80.
Trabbic-Carlson, Kimberly, et al. “Swelling and mechanical behaviors of chemically cross-linked hydrogels of elastin-like polypeptides.Biomacromolecules, vol. 4, no. 3, May 2003, pp. 572–80. Epmc, doi:10.1021/bm025671z.
Trabbic-Carlson K, Setton LA, Chilkoti A. Swelling and mechanical behaviors of chemically cross-linked hydrogels of elastin-like polypeptides. Biomacromolecules. 2003 May;4(3):572–580.
Journal cover image

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

May 2003

Volume

4

Issue

3

Start / End Page

572 / 580

Related Subject Headings

  • Thermodynamics
  • Temperature
  • Stress, Mechanical
  • Polymers
  • Peptides
  • Molecular Weight
  • Hydrogels
  • Cross-Linking Reagents
  • 40 Engineering
  • 34 Chemical sciences