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Synergistic effect of shear stress and streptavidin-biotin on the expression of endothelial vasodilator and cytoskeleton genes.

Publication ,  Journal Article
Chan, BP; Reichert, WM; Truskey, GA
Published in: Biotechnology and bioengineering
December 2004

Dual ligand treatment of streptavidin(SA)-biotin and fibronectin (Fn) enhances the adhesion of endothelial cells (EC) onto synthetic surfaces and promotes the quiescent phenotype of adherent EC. The current study investigates the effect of the dual ligand on the expression of endothelial genes in static culture and under shear stress (4 h at 10 dynes/cm2). Expression of 23 genes in the classes of signaling, cytoskeleton/ECM, vasoregulation, and shear-responsive were examined. Eight genes (argininosuccinate synthetase, K+ channel, TGFbeta, Mn-SOD, alpha-tubulin, t-PA, COX2, and eNOS) were significantly upregulated by shear stress. Two genes (caveolin-1 and ET-1) were downregulated by shear stress. Three genes (RhoA, elastin, alpha-actinin) were upregulated by the dual ligand treatment in static culture, and four genes (FAK, elastin, COX2, and eNOS) were upregulated when the dual ligand and shear stress were applied simultaneously. Northern blot analyses on FAK, RhoA, elastin, and alpha-actinin revealed similar results. The results suggest (1) the use of SA-biotin to supplement EC adhesion enhances the integrity of the EC cytoskeleton by upregulating the expression of cytoskeleton/ECM genes, and (2) a likely relationship between the expression of cytoskeleton/ECM genes and the downstream events, such as the shear-induced expression of eNOS and COX2 genes. Analyses presented in this study provide insights into the mechanism by which SA-biotin-supplemented EC mediate gene expression.

Duke Scholars

Published In

Biotechnology and bioengineering

DOI

EISSN

1097-0290

ISSN

0006-3592

Publication Date

December 2004

Volume

88

Issue

6

Start / End Page

750 / 758

Related Subject Headings

  • Vasodilation
  • Tissue Engineering
  • Stress, Mechanical
  • Streptavidin
  • Shear Strength
  • Mechanotransduction, Cellular
  • Humans
  • Gene Expression Regulation
  • Fibronectins
  • Endothelial Cells
 

Citation

APA
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ICMJE
MLA
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Chan, B. P., Reichert, W. M., & Truskey, G. A. (2004). Synergistic effect of shear stress and streptavidin-biotin on the expression of endothelial vasodilator and cytoskeleton genes. Biotechnology and Bioengineering, 88(6), 750–758. https://doi.org/10.1002/bit.20263
Chan, Bernard P., William M. Reichert, and George A. Truskey. “Synergistic effect of shear stress and streptavidin-biotin on the expression of endothelial vasodilator and cytoskeleton genes.Biotechnology and Bioengineering 88, no. 6 (December 2004): 750–58. https://doi.org/10.1002/bit.20263.
Chan BP, Reichert WM, Truskey GA. Synergistic effect of shear stress and streptavidin-biotin on the expression of endothelial vasodilator and cytoskeleton genes. Biotechnology and bioengineering. 2004 Dec;88(6):750–8.
Chan, Bernard P., et al. “Synergistic effect of shear stress and streptavidin-biotin on the expression of endothelial vasodilator and cytoskeleton genes.Biotechnology and Bioengineering, vol. 88, no. 6, Dec. 2004, pp. 750–58. Epmc, doi:10.1002/bit.20263.
Chan BP, Reichert WM, Truskey GA. Synergistic effect of shear stress and streptavidin-biotin on the expression of endothelial vasodilator and cytoskeleton genes. Biotechnology and bioengineering. 2004 Dec;88(6):750–758.
Journal cover image

Published In

Biotechnology and bioengineering

DOI

EISSN

1097-0290

ISSN

0006-3592

Publication Date

December 2004

Volume

88

Issue

6

Start / End Page

750 / 758

Related Subject Headings

  • Vasodilation
  • Tissue Engineering
  • Stress, Mechanical
  • Streptavidin
  • Shear Strength
  • Mechanotransduction, Cellular
  • Humans
  • Gene Expression Regulation
  • Fibronectins
  • Endothelial Cells