Dynamic response of endothelial cells to flow as measured by total internal reflectance fluorescence microscopy
Publication
, Journal Article
Olivier, LA; Reichert, WM; Truskey, GA
Published in: American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
January 1, 1995
To study the effect of luminal fluid stress on the abluminal cell surface and its adhesion sites, called focal contacts, total internal fluorescence reflectance microscopy is developed for measuring the dynamic response of endothelial cell focal contacts to flow in vitro. This method is used to illuminate the lower cell membrane so that focal contact movement can be followed over time. Based on the results, it is indicated that abluminal cell contour modulation occurs at a moderate level under static and low shear conditions, increasing substantially at higher shear rates. Moreover, at early flow time, the cell contour is moving away from the surface and focal contact sites are decreasing in response to the fluid stress.
Duke Scholars
Published In
American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Publication Date
January 1, 1995
Volume
29
Start / End Page
565 / 566
Related Subject Headings
- Biomedical Engineering
Citation
APA
Chicago
ICMJE
MLA
NLM
Olivier, L. A., Reichert, W. M., & Truskey, G. A. (1995). Dynamic response of endothelial cells to flow as measured by total internal reflectance fluorescence microscopy. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED, 29, 565–566.
Olivier, L. A., W. M. Reichert, and G. A. Truskey. “Dynamic response of endothelial cells to flow as measured by total internal reflectance fluorescence microscopy.” American Society of Mechanical Engineers, Bioengineering Division (Publication) BED 29 (January 1, 1995): 565–66.
Olivier LA, Reichert WM, Truskey GA. Dynamic response of endothelial cells to flow as measured by total internal reflectance fluorescence microscopy. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. 1995 Jan 1;29:565–6.
Olivier, L. A., et al. “Dynamic response of endothelial cells to flow as measured by total internal reflectance fluorescence microscopy.” American Society of Mechanical Engineers, Bioengineering Division (Publication) BED, vol. 29, Jan. 1995, pp. 565–66.
Olivier LA, Reichert WM, Truskey GA. Dynamic response of endothelial cells to flow as measured by total internal reflectance fluorescence microscopy. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. 1995 Jan 1;29:565–566.
Published In
American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Publication Date
January 1, 1995
Volume
29
Start / End Page
565 / 566
Related Subject Headings
- Biomedical Engineering