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A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface.

Publication ,  Journal Article
Nath, N; Chilkoti, A
Published in: Analytical chemistry
February 2002

This paper presents a new label-free optical method to study biomolecular interactions in real time at the surface of an optically transparent substrate. The method relies on the change in the absorbance spectrum of a self-assembled monolayer of colloidal gold on glass, as a function of biomolecular binding to the surface of the immobilized colloids. Using this approach, we demonstrate proof of principle of a label-free optical biosensor to quantify biomolecular interactions in real time on a surface in a commercially available UV-visible spectrophotometer and of a colorimetric end-point assay using an optical scanner. The spectrophotometric sensor shows concentration-dependent binding and a detection limit of 16 nM for streptavidin. The sensor is easy to fabricate, is reproducible in its performance, has minimal technological requirements, namely, the availability of an UV-visible spectrophotometer or an optical scanner, and will enable high-throughput screening of biomolecular interactions in real time in an array-based format.

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Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

February 2002

Volume

74

Issue

3

Start / End Page

504 / 509

Related Subject Headings

  • Streptavidin
  • Spectrum Analysis
  • Sensitivity and Specificity
  • Nanotechnology
  • Ligands
  • Gold Colloid
  • Biotin
  • Biosensing Techniques
  • Analytical Chemistry
  • 4004 Chemical engineering
 

Citation

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Nath, N., & Chilkoti, A. (2002). A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface. Analytical Chemistry, 74(3), 504–509. https://doi.org/10.1021/ac015657x
Nath, Nidhi, and Ashutosh Chilkoti. “A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface.Analytical Chemistry 74, no. 3 (February 2002): 504–9. https://doi.org/10.1021/ac015657x.
Nath, Nidhi, and Ashutosh Chilkoti. “A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface.Analytical Chemistry, vol. 74, no. 3, Feb. 2002, pp. 504–09. Epmc, doi:10.1021/ac015657x.
Journal cover image

Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

February 2002

Volume

74

Issue

3

Start / End Page

504 / 509

Related Subject Headings

  • Streptavidin
  • Spectrum Analysis
  • Sensitivity and Specificity
  • Nanotechnology
  • Ligands
  • Gold Colloid
  • Biotin
  • Biosensing Techniques
  • Analytical Chemistry
  • 4004 Chemical engineering