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Dynamic addressing of a surface pattern by a stimuli-responsive fusion protein

Publication ,  Journal Article
Frey, W; Meyer, DE; Chilkoti, A
Published in: Advanced Materials
February 5, 2003

An overview is given of a dynamic protein patterning methodology, thermodynamically reversible addressing of proteins (TRAP), in which a recombinant, stimuli responsive polypeptide is used to dynamically address a fusion protein onto a micropatterned surface template by modulating the interaction between a chemically patterned surface and the stimuli-responsive polypeptide. Immobilization of the fusion protein in spatially defined regions of the micropatterned surface is triggered either by an increase in temperature or ionic strength and the immobilized fusion partner is oriented towards solution and is recognized by an antibody to the fusion protein. TRAP is reversible, because the protein-antibody complex can be resolubilized from the surface by reversing the environmental trigger.

Duke Scholars

Published In

Advanced Materials

DOI

ISSN

0935-9648

Publication Date

February 5, 2003

Volume

15

Issue

3

Start / End Page

248 / 251

Related Subject Headings

  • Nanoscience & Nanotechnology
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
  • 03 Chemical Sciences
  • 02 Physical Sciences
 

Citation

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Frey, W., Meyer, D. E., & Chilkoti, A. (2003). Dynamic addressing of a surface pattern by a stimuli-responsive fusion protein. Advanced Materials, 15(3), 248–251. https://doi.org/10.1002/adma.200390058
Frey, W., D. E. Meyer, and A. Chilkoti. “Dynamic addressing of a surface pattern by a stimuli-responsive fusion protein.” Advanced Materials 15, no. 3 (February 5, 2003): 248–51. https://doi.org/10.1002/adma.200390058.
Frey W, Meyer DE, Chilkoti A. Dynamic addressing of a surface pattern by a stimuli-responsive fusion protein. Advanced Materials. 2003 Feb 5;15(3):248–51.
Frey, W., et al. “Dynamic addressing of a surface pattern by a stimuli-responsive fusion protein.” Advanced Materials, vol. 15, no. 3, Feb. 2003, pp. 248–51. Scopus, doi:10.1002/adma.200390058.
Frey W, Meyer DE, Chilkoti A. Dynamic addressing of a surface pattern by a stimuli-responsive fusion protein. Advanced Materials. 2003 Feb 5;15(3):248–251.
Journal cover image

Published In

Advanced Materials

DOI

ISSN

0935-9648

Publication Date

February 5, 2003

Volume

15

Issue

3

Start / End Page

248 / 251

Related Subject Headings

  • Nanoscience & Nanotechnology
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
  • 03 Chemical Sciences
  • 02 Physical Sciences