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Really smart bioconjugates of smart polymers and receptor proteins

Publication ,  Journal Article
Hoffman, AS; Stayton, PS; Bulmus, V; Chen, G; Chen, J; Cheung, C; Chilkoti, A; Ding, Z; Dong, L; Fong, R; Lackey, CA; Long, CJ; Miura, M ...
Published in: Journal of Biomedical Materials Research
2000

Over the past 18 years we have been deeply involved with the synthesis and applications of stimuliresponsive polymer systems, especially polymer-biomolecule conjugates. This article summarizes our work with one of these conjugate systems, specifically polymer-protein conjugates. We include conjugates prepared by random polymer conjugation to lysine amino groups, and also those prepared by site-specific conjugation of the polymer to specific amino acid sites that are genetically engineered into the known amino acid sequence of the protein. We describe the preparation and properties of thermally sensitive random conjugates to enzymes and several affinity recognition proteins. We have also prepared site-specific conjugates to streptavidin with temperature-sensitive polymers, pH-sensitive polymers, and light-sensitive polymers. The preparation of these conjugates and their many fascinating applications are reviewed in this article.

Duke Scholars

Published In

Journal of Biomedical Materials Research

DOI

Publication Date

2000

Volume

52

Issue

4

Start / End Page

577 / 586

Related Subject Headings

  • Biomedical Engineering
  • 0912 Materials Engineering
  • 0903 Biomedical Engineering
 

Citation

APA
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ICMJE
MLA
NLM
Hoffman, A. S., Stayton, P. S., Bulmus, V., Chen, G., Chen, J., Cheung, C., … al, E. (2000). Really smart bioconjugates of smart polymers and receptor proteins. Journal of Biomedical Materials Research, 52(4), 577–586. https://doi.org/10.1002/1097-4636(20001215)52:43.0.CO;2-5
Hoffman, Allan S., Patrick S. Stayton, Volga Bulmus, Guohua Chen, Jingping Chen, Chuck Cheung, Ashutosh Chilkoti, et al. “Really smart bioconjugates of smart polymers and receptor proteins.” Journal of Biomedical Materials Research 52, no. 4 (2000): 577–86. https://doi.org/10.1002/1097-4636(20001215)52:43.0.CO;2-5.
Hoffman AS, Stayton PS, Bulmus V, Chen G, Chen J, Cheung C, et al. Really smart bioconjugates of smart polymers and receptor proteins. Journal of Biomedical Materials Research. 2000;52(4):577–86.
Hoffman, Allan S., et al. “Really smart bioconjugates of smart polymers and receptor proteins.” Journal of Biomedical Materials Research, vol. 52, no. 4, 2000, pp. 577–86. Manual, doi:10.1002/1097-4636(20001215)52:43.0.CO;2-5.
Hoffman AS, Stayton PS, Bulmus V, Chen G, Chen J, Cheung C, Chilkoti A, Ding Z, Dong L, Fong R, Lackey CA, Long CJ, Miura M, Morris JE, Murthy N, al E. Really smart bioconjugates of smart polymers and receptor proteins. Journal of Biomedical Materials Research. 2000;52(4):577–586.

Published In

Journal of Biomedical Materials Research

DOI

Publication Date

2000

Volume

52

Issue

4

Start / End Page

577 / 586

Related Subject Headings

  • Biomedical Engineering
  • 0912 Materials Engineering
  • 0903 Biomedical Engineering