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Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption

Publication ,  Journal Article
Zhang, Z; Ma, H; Hausner, DB; Chilkoti, A; Beebe Jr, TP
Published in: Biomacromolecules
2005

New applications in regenerative biotechnology require the ability to understand and control protein- surface interactions on micrometer and submicrometer length scales. Evidence presented here shows that micropatterned amphiphilic comb polymer films exhibit a pretreatment-dependent behavior with respect to protein adsorption for the proteins fibronectin, laminin, and for serum. A micropatterned surface, consisting of protein-reactive regions, separated by comb polymer, was created and tested for protein adsorption using the surface-sensitive imaging tool TOF-SIMS. Immersion of micropatterned surfaces in solutions of fibronectin or laminin resulted in uniform protein coverage on both the comb polymer and protein-reactive regions. However, preimmersion of similarly patterned surfaces in water for 2 h prior to protein incubation was found to dramatically improve the protein-resistant properties of the comb polymer regions. These results are consistent with poly(ethylene glycol) (PEG) side chain reorientation and/or hydration and poly-(methyl methacrylate) (PMMA) backbone segregation away from the interface region. © 2005 American Chemical Society.

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Published In

Biomacromolecules

DOI

Publication Date

2005

Volume

6

Issue

6

Start / End Page

3388 / 3396

Related Subject Headings

  • Time Factors
  • Surface Properties
  • Proteins
  • Polymethyl Methacrylate
  • Polymers
  • Polymers
  • Polyethylene Glycols
  • Molecular Structure
  • Models, Chemical
  • Microscopy, Atomic Force
 

Citation

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Zhang, Z., Ma, H., Hausner, D. B., Chilkoti, A., & Beebe Jr, T. P. (2005). Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption. Biomacromolecules, 6(6), 3388–3396. https://doi.org/10.1021/bm050446d
Zhang, Zhanping, Hongwei Ma, Douglas B. Hausner, Ashutosh Chilkoti, and Thomas P. Beebe Jr. “Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption.” Biomacromolecules 6, no. 6 (2005): 3388–96. https://doi.org/10.1021/bm050446d.
Zhang Z, Ma H, Hausner DB, Chilkoti A, Beebe Jr TP. Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption. Biomacromolecules. 2005;6(6):3388–96.
Zhang, Zhanping, et al. “Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption.” Biomacromolecules, vol. 6, no. 6, 2005, pp. 3388–96. Manual, doi:10.1021/bm050446d.
Zhang Z, Ma H, Hausner DB, Chilkoti A, Beebe Jr TP. Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption. Biomacromolecules. 2005;6(6):3388–3396.

Published In

Biomacromolecules

DOI

Publication Date

2005

Volume

6

Issue

6

Start / End Page

3388 / 3396

Related Subject Headings

  • Time Factors
  • Surface Properties
  • Proteins
  • Polymethyl Methacrylate
  • Polymers
  • Polymers
  • Polyethylene Glycols
  • Molecular Structure
  • Models, Chemical
  • Microscopy, Atomic Force