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Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size.

Publication ,  Journal Article
Nath, N; Chilkoti, A
Published in: Analytical chemistry
September 2004

The unique optical properties of noble metal nanoparticles have been used to design a label-free biosensor in a chip format. In this paper, we demonstrate that the size of gold nanoparticles significantly affects the sensitivity of the biosensor. Gold nanoparticles with diameters in the range of 12-48 nm were synthesized in solution and sensor chips were fabricated by chemisorption of these nanoparticles on amine-functionalized glass. Sensors fabricated from 39-nm-diameter gold nanoparticles exhibited maximum sensitivity to the change of the bulk refractive index and the largest "analytical volume", defined as the region around the nanoparticle within which a change in refractive index causes a change in the optical properties of the immobilized nanoparticles. The detection limit for streptavidin-biotin binding of a sensor fabricated from 39-nm-diameter nanoparticles was 20-fold better than a previously reported sensor fabricated from 13-nm-diameter gold nanoparticles. We also discuss several other factors that could improve the performance of the next generation of these immobilized metal nanoparticle sensors.

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Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

September 2004

Volume

76

Issue

18

Start / End Page

5370 / 5378

Related Subject Headings

  • Surface Plasmon Resonance
  • Spectrum Analysis
  • Sensitivity and Specificity
  • Refractometry
  • Particle Size
  • Nanostructures
  • Microscopy, Atomic Force
  • Gold
  • Glass
  • Analytical Chemistry
 

Citation

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Nath, N., & Chilkoti, A. (2004). Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size. Analytical Chemistry, 76(18), 5370–5378. https://doi.org/10.1021/ac049741z
Nath, Nidhi, and Ashutosh Chilkoti. “Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size.Analytical Chemistry 76, no. 18 (September 2004): 5370–78. https://doi.org/10.1021/ac049741z.
Nath, Nidhi, and Ashutosh Chilkoti. “Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size.Analytical Chemistry, vol. 76, no. 18, Sept. 2004, pp. 5370–78. Epmc, doi:10.1021/ac049741z.
Journal cover image

Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

September 2004

Volume

76

Issue

18

Start / End Page

5370 / 5378

Related Subject Headings

  • Surface Plasmon Resonance
  • Spectrum Analysis
  • Sensitivity and Specificity
  • Refractometry
  • Particle Size
  • Nanostructures
  • Microscopy, Atomic Force
  • Gold
  • Glass
  • Analytical Chemistry