A digital microfluidic biosensor for multianalyte detection
Publication
, Journal Article
Srinivasan, V; Pamula, V; Pollack, M; Fair, R
Published in: Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS)
July 23, 2003
A microfluidic lab-on-chip (LoC) for measuring the concentration of human body metabolites, using submicroliter droplets as reaction chambers, is presented in this paper. The device is based on the manipulation of droplets using the principle of electrowetting and is integrated with an optical absorbance measurement system for detection. We have demonstrated the detection of glucose using a colorimetric enzyme-kinetic assay in less than 40 seconds. The sensor response is linear in the range of 25mg/dl to 300mg/dl, with less than 5% deviation from linearity at the upper limit. In addition to glucose, we have also demonstrated the feasibility of lactate, glutamate, and pyruvate assays using our system.
Duke Scholars
Published In
Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS)
Publication Date
July 23, 2003
Start / End Page
327 / 330
Citation
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MLA
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Srinivasan, V., Pamula, V., Pollack, M., & Fair, R. (2003). A digital microfluidic biosensor for multianalyte detection. Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS), 327–330.
Srinivasan, V., V. Pamula, M. Pollack, and R. Fair. “A digital microfluidic biosensor for multianalyte detection.” Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS), July 23, 2003, 327–30.
Srinivasan V, Pamula V, Pollack M, Fair R. A digital microfluidic biosensor for multianalyte detection. Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS). 2003 Jul 23;327–30.
Srinivasan, V., et al. “A digital microfluidic biosensor for multianalyte detection.” Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS), July 2003, pp. 327–30.
Srinivasan V, Pamula V, Pollack M, Fair R. A digital microfluidic biosensor for multianalyte detection. Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS). 2003 Jul 23;327–330.
Published In
Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS)
Publication Date
July 23, 2003
Start / End Page
327 / 330