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Differential seizure sensitivities to picrotoxinin in two inbred strains of mice (DBA/2J and BALB/c ByJ): parallel changes in GABA receptor-mediated chloride flux and receptor binding.

Publication ,  Journal Article
Schwartz, RD; Seale, TW; Skolnick, P; Paul, SM
Published in: Brain Res
February 27, 1989

Two strains of mice were shown to possess a differential sensitivity to picrotoxinin-induced convulsions; picrotoxinin elicited both tonic and clonic seizures at lower doses in the DBA/2J (DBA) strain compared to the BALB/c ByJ (BALB) strain. Less protection of picrotoxinin-induced tonic seizures was afforded by pentobarbital in the DBA strain. Biochemical studies revealed that picrotoxin inhibited 36Cl- efflux from forebrain synaptoneurosomes only in the DBA strain. In addition, picrotoxin inhibited pentobarbital-induced 36Cl- efflux to a greater extent in the DBA strain. No differences were observed in the binding of [3H]muscimol or [35S]t-butylbicyclophosphorothionate (TBPS) to forebrain homogenates, while pentobarbital was a less potent inhibitor of [35S]TBPS binding in the DBA strain. These findings suggest a genetic basis for the behavioral differences in convulsant sensitivity as well as for the neurochemical differences in allosteric coupling between convulsant and depressant/anticonvulsant sites associated with the GABA receptor-gated Cl- channel.

Duke Scholars

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

February 27, 1989

Volume

481

Issue

1

Start / End Page

169 / 174

Location

Netherlands

Related Subject Headings

  • Synaptosomes
  • Species Specificity
  • Seizures
  • Receptors, GABA-A
  • Picrotoxin
  • Pentobarbital
  • Neurology & Neurosurgery
  • Muscimol
  • Mice, Inbred DBA
  • Mice, Inbred BALB C
 

Citation

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Schwartz, R. D., Seale, T. W., Skolnick, P., & Paul, S. M. (1989). Differential seizure sensitivities to picrotoxinin in two inbred strains of mice (DBA/2J and BALB/c ByJ): parallel changes in GABA receptor-mediated chloride flux and receptor binding. Brain Res, 481(1), 169–174. https://doi.org/10.1016/0006-8993(89)90499-x
Schwartz, R. D., T. W. Seale, P. Skolnick, and S. M. Paul. “Differential seizure sensitivities to picrotoxinin in two inbred strains of mice (DBA/2J and BALB/c ByJ): parallel changes in GABA receptor-mediated chloride flux and receptor binding.Brain Res 481, no. 1 (February 27, 1989): 169–74. https://doi.org/10.1016/0006-8993(89)90499-x.
Schwartz, R. D., et al. “Differential seizure sensitivities to picrotoxinin in two inbred strains of mice (DBA/2J and BALB/c ByJ): parallel changes in GABA receptor-mediated chloride flux and receptor binding.Brain Res, vol. 481, no. 1, Feb. 1989, pp. 169–74. Pubmed, doi:10.1016/0006-8993(89)90499-x.
Journal cover image

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

February 27, 1989

Volume

481

Issue

1

Start / End Page

169 / 174

Location

Netherlands

Related Subject Headings

  • Synaptosomes
  • Species Specificity
  • Seizures
  • Receptors, GABA-A
  • Picrotoxin
  • Pentobarbital
  • Neurology & Neurosurgery
  • Muscimol
  • Mice, Inbred DBA
  • Mice, Inbred BALB C