A physical map of the mouse genome.
A physical map of a genome is an essential guide for navigation, allowing the location of any gene or other landmark in the chromosomal DNA. We have constructed a physical map of the mouse genome that contains 296 contigs of overlapping bacterial clones and 16,992 unique markers. The mouse contigs were aligned to the human genome sequence on the basis of 51,486 homology matches, thus enabling use of the conserved synteny (correspondence between chromosome blocks) of the two genomes to accelerate construction of the mouse map. The map provides a framework for assembly of whole-genome shotgun sequence data, and a tile path of clones for generation of the reference sequence. Definition of the human-mouse alignment at this level of resolution enables identification of a mouse clone that corresponds to almost any position in the human genome. The human sequence may be used to facilitate construction of other mammalian genome maps using the same strategy.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Synteny
- Species Specificity
- Sequence Homology, Nucleic Acid
- Sequence Alignment
- Radiation Hybrid Mapping
- Physical Chromosome Mapping
- Molecular Sequence Data
- Mice
- Humans
- Genome, Human
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Synteny
- Species Specificity
- Sequence Homology, Nucleic Acid
- Sequence Alignment
- Radiation Hybrid Mapping
- Physical Chromosome Mapping
- Molecular Sequence Data
- Mice
- Humans
- Genome, Human