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Identification of a structurally distinct CD101 molecule encoded in the 950-kb Idd10 region of NOD mice.

Publication ,  Journal Article
Penha-Gonçalves, C; Moule, C; Smink, LJ; Howson, J; Gregory, S; Rogers, J; Lyons, PA; Suttie, JJ; Lord, CJ; Peterson, LB; Todd, JA; Wicker, LS
Published in: Diabetes
June 2003

Genes affecting autoimmune type 1 diabetes susceptibility in the nonobese diabetic (NOD) mouse (Idd loci) have been mapped using a congenic strain breeding strategy. In the present study, we used a combination of BAC clone contig construction, polymorphism analysis of DNA from congenic strains, and sequence mining of the human orthologous region to generate an integrated map of the Idd10 region on mouse chromosome 3. We found seven genes and one pseudogene in the 950-kb Idd10 region. Although all seven genes in the interval are Idd10 candidates, we suggest the gene encoding the EWI immunoglobulin subfamily member EWI-101 (Cd101) as the most likely Idd10 candidate because of the previously reported immune-associated properties of the human CD101 molecule. Additional support for the candidacy of Cd101 is the presence of 17 exonic single-nucleotide polymorphisms that differ between the NOD and B6 sequences, 10 causing amino acid substitutions in the predicted CD101 protein. Four of these 10 substitutions are nonconservative, 2 of which could potentially alter N-linked glycosylation. Considering our results together with those previous reports that antibodies recognizing human CD101 modulate human T-cell and dendritic cell function, there is now justification to test whether the alteration of CD101 function affects autoimmune islet destruction.

Duke Scholars

Published In

Diabetes

DOI

ISSN

0012-1797

Publication Date

June 2003

Volume

52

Issue

6

Start / End Page

1551 / 1556

Location

United States

Related Subject Headings

  • Pseudogenes
  • Polymorphism, Single Nucleotide
  • Molecular Sequence Data
  • Mice, Inbred NOD
  • Mice
  • Membrane Glycoproteins
  • Genetic Variation
  • Genetic Predisposition to Disease
  • Genetic Markers
  • Exons
 

Citation

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Penha-Gonçalves, C., Moule, C., Smink, L. J., Howson, J., Gregory, S., Rogers, J., … Wicker, L. S. (2003). Identification of a structurally distinct CD101 molecule encoded in the 950-kb Idd10 region of NOD mice. Diabetes, 52(6), 1551–1556. https://doi.org/10.2337/diabetes.52.6.1551
Penha-Gonçalves, Carlos, Carolyn Moule, Luc J. Smink, Joanna Howson, Simon Gregory, Jane Rogers, Paul A. Lyons, et al. “Identification of a structurally distinct CD101 molecule encoded in the 950-kb Idd10 region of NOD mice.Diabetes 52, no. 6 (June 2003): 1551–56. https://doi.org/10.2337/diabetes.52.6.1551.
Penha-Gonçalves C, Moule C, Smink LJ, Howson J, Gregory S, Rogers J, et al. Identification of a structurally distinct CD101 molecule encoded in the 950-kb Idd10 region of NOD mice. Diabetes. 2003 Jun;52(6):1551–6.
Penha-Gonçalves, Carlos, et al. “Identification of a structurally distinct CD101 molecule encoded in the 950-kb Idd10 region of NOD mice.Diabetes, vol. 52, no. 6, June 2003, pp. 1551–56. Pubmed, doi:10.2337/diabetes.52.6.1551.
Penha-Gonçalves C, Moule C, Smink LJ, Howson J, Gregory S, Rogers J, Lyons PA, Suttie JJ, Lord CJ, Peterson LB, Todd JA, Wicker LS. Identification of a structurally distinct CD101 molecule encoded in the 950-kb Idd10 region of NOD mice. Diabetes. 2003 Jun;52(6):1551–1556.

Published In

Diabetes

DOI

ISSN

0012-1797

Publication Date

June 2003

Volume

52

Issue

6

Start / End Page

1551 / 1556

Location

United States

Related Subject Headings

  • Pseudogenes
  • Polymorphism, Single Nucleotide
  • Molecular Sequence Data
  • Mice, Inbred NOD
  • Mice
  • Membrane Glycoproteins
  • Genetic Variation
  • Genetic Predisposition to Disease
  • Genetic Markers
  • Exons