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Mapping and characterization of the amplicon near APOA2 in 1q23 in human sarcomas by FISH and array CGH.

Publication ,  Journal Article
Kresse, SH; Berner, J-M; Meza-Zepeda, LA; Gregory, SG; Kuo, W-L; Gray, JW; Forus, A; Myklebost, O
Published in: Mol Cancer
November 7, 2005

BACKGROUND: Amplification of the q21-q23 region on chromosome 1 is frequently found in sarcomas and a variety of other solid tumours. Previous analyses of sarcomas have indicated the presence of at least two separate amplicons within this region, one located in 1q21 and one located near the apolipoprotein A-II (APOA2) gene in 1q23. In this study we have mapped and characterized the amplicon in 1q23 in more detail. RESULTS: We have used fluorescence in situ hybridisation (FISH) and microarray-based comparative genomic hybridisation (array CGH) to map and define the borders of the amplicon in 10 sarcomas. A subregion of approximately 800 kb was identified as the core of the amplicon. The amplification patterns of nine possible candidate target genes located to this subregion were determined by Southern blot analysis. The genes activating transcription factor 6 (ATF6) and dual specificity phosphatase 12 (DUSP12) showed the highest level of amplification, and they were also shown to be over-expressed by quantitative real-time reverse transcription PCR (RT-PCR). In general, the level of expression reflected the level of amplification in the different tumours. DUSP12 was expressed significantly higher than ATF6 in a subset of the tumours. In addition, two genes known to be transcriptionally activated by ATF6, glucose-regulated protein 78 kDa and -94 kDa (GRP78 and GRP94), were shown to be over-expressed in the tumours that showed over-expression of ATF6. CONCLUSION: ATF6 and DUSP12 seem to be the most likely candidate target genes for the 1q23 amplification in sarcomas. Both genes have possible roles in promoting cell growth, which makes them interesting candidate targets.

Duke Scholars

Published In

Mol Cancer

DOI

EISSN

1476-4598

Publication Date

November 7, 2005

Volume

4

Start / End Page

39

Location

England

Related Subject Headings

  • Sarcoma
  • RNA, Messenger
  • Physical Chromosome Mapping
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Nucleic Acid Hybridization
  • In Situ Hybridization, Fluorescence
  • Humans
  • Genome, Human
  • Gene Expression Regulation, Neoplastic
 

Citation

APA
Chicago
ICMJE
MLA
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Kresse, S. H., Berner, J.-M., Meza-Zepeda, L. A., Gregory, S. G., Kuo, W.-L., Gray, J. W., … Myklebost, O. (2005). Mapping and characterization of the amplicon near APOA2 in 1q23 in human sarcomas by FISH and array CGH. Mol Cancer, 4, 39. https://doi.org/10.1186/1476-4598-4-39
Kresse, Stine H., Jeanne-Marie Berner, Leonardo A. Meza-Zepeda, Simon G. Gregory, Wen-Lin Kuo, Joe W. Gray, Anne Forus, and Ola Myklebost. “Mapping and characterization of the amplicon near APOA2 in 1q23 in human sarcomas by FISH and array CGH.Mol Cancer 4 (November 7, 2005): 39. https://doi.org/10.1186/1476-4598-4-39.
Kresse SH, Berner J-M, Meza-Zepeda LA, Gregory SG, Kuo W-L, Gray JW, et al. Mapping and characterization of the amplicon near APOA2 in 1q23 in human sarcomas by FISH and array CGH. Mol Cancer. 2005 Nov 7;4:39.
Kresse, Stine H., et al. “Mapping and characterization of the amplicon near APOA2 in 1q23 in human sarcomas by FISH and array CGH.Mol Cancer, vol. 4, Nov. 2005, p. 39. Pubmed, doi:10.1186/1476-4598-4-39.
Kresse SH, Berner J-M, Meza-Zepeda LA, Gregory SG, Kuo W-L, Gray JW, Forus A, Myklebost O. Mapping and characterization of the amplicon near APOA2 in 1q23 in human sarcomas by FISH and array CGH. Mol Cancer. 2005 Nov 7;4:39.
Journal cover image

Published In

Mol Cancer

DOI

EISSN

1476-4598

Publication Date

November 7, 2005

Volume

4

Start / End Page

39

Location

England

Related Subject Headings

  • Sarcoma
  • RNA, Messenger
  • Physical Chromosome Mapping
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Nucleic Acid Hybridization
  • In Situ Hybridization, Fluorescence
  • Humans
  • Genome, Human
  • Gene Expression Regulation, Neoplastic