The study of protein mechanics with the atomic force microscope.
Publication
, Journal Article
Fisher, TE; Oberhauser, AF; Carrion-Vazquez, M; Marszalek, PE; Fernandez, JM
Published in: Trends in biochemical sciences
October 1999
The unfolding and folding of single protein molecules can be studied with an atomic force microscope (AFM). Many proteins with mechanical functions contain multiple, individually folded domains with similar structures. Protein engineering techniques have enabled the construction and expression of recombinant proteins that contain multiple copies of identical domains. Thus, the AFM in combination with protein engineering has enabled the kinetic analysis of the force-induced unfolding and refolding of individual domains as well as the study of the determinants of mechanical stability.
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Published In
Trends in biochemical sciences
DOI
ISSN
0968-0004
Publication Date
October 1999
Volume
24
Issue
10
Start / End Page
379 / 384
Related Subject Headings
- Proteins
- Protein Folding
- Protein Binding
- Microscopy, Atomic Force
- Kinetics
- Entropy
- Developmental Biology
- 3404 Medicinal and biomolecular chemistry
- 3205 Medical biochemistry and metabolomics
- 3101 Biochemistry and cell biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Fisher, T. E., Oberhauser, A. F., Carrion-Vazquez, M., Marszalek, P. E., & Fernandez, J. M. (1999). The study of protein mechanics with the atomic force microscope. Trends in Biochemical Sciences, 24(10), 379–384. https://doi.org/10.1016/s0968-0004(99)01453-x
Fisher, T. E., A. F. Oberhauser, M. Carrion-Vazquez, P. E. Marszalek, and J. M. Fernandez. “The study of protein mechanics with the atomic force microscope.” Trends in Biochemical Sciences 24, no. 10 (October 1999): 379–84. https://doi.org/10.1016/s0968-0004(99)01453-x.
Fisher TE, Oberhauser AF, Carrion-Vazquez M, Marszalek PE, Fernandez JM. The study of protein mechanics with the atomic force microscope. Trends in biochemical sciences. 1999 Oct;24(10):379–84.
Fisher, T. E., et al. “The study of protein mechanics with the atomic force microscope.” Trends in Biochemical Sciences, vol. 24, no. 10, Oct. 1999, pp. 379–84. Epmc, doi:10.1016/s0968-0004(99)01453-x.
Fisher TE, Oberhauser AF, Carrion-Vazquez M, Marszalek PE, Fernandez JM. The study of protein mechanics with the atomic force microscope. Trends in biochemical sciences. 1999 Oct;24(10):379–384.
Published In
Trends in biochemical sciences
DOI
ISSN
0968-0004
Publication Date
October 1999
Volume
24
Issue
10
Start / End Page
379 / 384
Related Subject Headings
- Proteins
- Protein Folding
- Protein Binding
- Microscopy, Atomic Force
- Kinetics
- Entropy
- Developmental Biology
- 3404 Medicinal and biomolecular chemistry
- 3205 Medical biochemistry and metabolomics
- 3101 Biochemistry and cell biology