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Microcantilever sensing and actuation with end-grafted stimulus-responsive elastin-like polypeptides.

Publication ,  Journal Article
Valiaev, A; Abu-Lail, NI; Lim, DW; Chilkoti, A; Zauscher, S
Published in: Langmuir : the ACS journal of surfaces and colloids
January 2007

Stimulus-responsive elastin-like polypeptides (ELPs) grafted onto surfaces are of significant technical interest because they can be exploited for force generation, in sensing applications, or as molecular switches with tunable properties. Changes in the conformational state of grafted ELPs, induced by a phase transition or changes in osmotic pressure, lead to significant changes in the surface stress in the ELP graft layer and translate into detectable changes in microcantilever deflection. In this study, we investigate the conformational mechanics of ELPs in response to changes in solution pH and ionic strength using atomic force microscopy (AFM) microcantilever deflection and quartz crystal microbalance (QCM) measurements. We show that the use of genetically encoded, surface-grafted ELPs is exciting for cantilever actuation and sensing because commonly available microfabricated cantilever springs offer a simple and nonintrusive way to detect changes in solvent type, temperature, and pH, promising great potential for sensing applications in microfluidic devices.

Duke Scholars

Published In

Langmuir : the ACS journal of surfaces and colloids

DOI

EISSN

1520-5827

ISSN

0743-7463

Publication Date

January 2007

Volume

23

Issue

1

Start / End Page

339 / 344

Related Subject Headings

  • Surface Properties
  • Stress, Mechanical
  • Protein Conformation
  • Nanotechnology
  • Hydrogen-Ion Concentration
  • Elastin
  • Chemical Physics
  • Biosensing Techniques
 

Citation

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Valiaev, A., Abu-Lail, N. I., Lim, D. W., Chilkoti, A., & Zauscher, S. (2007). Microcantilever sensing and actuation with end-grafted stimulus-responsive elastin-like polypeptides. Langmuir : The ACS Journal of Surfaces and Colloids, 23(1), 339–344. https://doi.org/10.1021/la0616698
Valiaev, Alexei, Nehal I. Abu-Lail, Dong Woo Lim, Ashutosh Chilkoti, and Stefan Zauscher. “Microcantilever sensing and actuation with end-grafted stimulus-responsive elastin-like polypeptides.Langmuir : The ACS Journal of Surfaces and Colloids 23, no. 1 (January 2007): 339–44. https://doi.org/10.1021/la0616698.
Valiaev A, Abu-Lail NI, Lim DW, Chilkoti A, Zauscher S. Microcantilever sensing and actuation with end-grafted stimulus-responsive elastin-like polypeptides. Langmuir : the ACS journal of surfaces and colloids. 2007 Jan;23(1):339–44.
Valiaev, Alexei, et al. “Microcantilever sensing and actuation with end-grafted stimulus-responsive elastin-like polypeptides.Langmuir : The ACS Journal of Surfaces and Colloids, vol. 23, no. 1, Jan. 2007, pp. 339–44. Epmc, doi:10.1021/la0616698.
Valiaev A, Abu-Lail NI, Lim DW, Chilkoti A, Zauscher S. Microcantilever sensing and actuation with end-grafted stimulus-responsive elastin-like polypeptides. Langmuir : the ACS journal of surfaces and colloids. 2007 Jan;23(1):339–344.
Journal cover image

Published In

Langmuir : the ACS journal of surfaces and colloids

DOI

EISSN

1520-5827

ISSN

0743-7463

Publication Date

January 2007

Volume

23

Issue

1

Start / End Page

339 / 344

Related Subject Headings

  • Surface Properties
  • Stress, Mechanical
  • Protein Conformation
  • Nanotechnology
  • Hydrogen-Ion Concentration
  • Elastin
  • Chemical Physics
  • Biosensing Techniques