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Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides.

Publication ,  Journal Article
Lim, DW; Trabbic-Carlson, K; Mackay, JA; Chilkoti, A
Published in: Biomacromolecules
May 2007

This paper reports an improvement in the purification of thioredoxin (Trx) expressed from E. coli by inverse transition cycling (ITC) using cationic elastin-like polypeptides (ELPs). Two ELP libraries having 2% and 5% lysine residues and molecular weights ranging from 4 to 61.1 kDa showed greater salt sensitivity in their inverse transition behavior than purely aliphatic ELPs. Expression yield of Trx-ELP fusions was an unpredictable function of guest residue composition, but reducing the molecular weight of the ELP tag generally increased Trx yield. A cationic 4.3 kDa ELP is the shortest ELP used to purify any protein by ITC to date. A 15.9 kDa ELP with a guest residue composition of K:V:F of 1:7:1 was found to be the optimal cationic tag to purify Trx, as it provided 50% greater Trx yield and only required one-fifth the added NaCl for purification of Trx as compared to previously used aliphatic ELP tags.

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Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

May 2007

Volume

8

Issue

5

Start / End Page

1417 / 1424

Related Subject Headings

  • Thioredoxins
  • Sodium Chloride
  • Recombinant Fusion Proteins
  • Polymers
  • Peptides
  • Peptide Library
  • Molecular Sequence Data
  • Escherichia coli
  • Elastin
  • Cations
 

Citation

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Lim, D. W., Trabbic-Carlson, K., Mackay, J. A., & Chilkoti, A. (2007). Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides. Biomacromolecules, 8(5), 1417–1424. https://doi.org/10.1021/bm060849t
Lim, Dong Woo, Kimberly Trabbic-Carlson, J Andrew Mackay, and Ashutosh Chilkoti. “Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides.Biomacromolecules 8, no. 5 (May 2007): 1417–24. https://doi.org/10.1021/bm060849t.
Lim DW, Trabbic-Carlson K, Mackay JA, Chilkoti A. Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides. Biomacromolecules. 2007 May;8(5):1417–24.
Lim, Dong Woo, et al. “Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides.Biomacromolecules, vol. 8, no. 5, May 2007, pp. 1417–24. Epmc, doi:10.1021/bm060849t.
Lim DW, Trabbic-Carlson K, Mackay JA, Chilkoti A. Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides. Biomacromolecules. 2007 May;8(5):1417–1424.
Journal cover image

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

May 2007

Volume

8

Issue

5

Start / End Page

1417 / 1424

Related Subject Headings

  • Thioredoxins
  • Sodium Chloride
  • Recombinant Fusion Proteins
  • Polymers
  • Peptides
  • Peptide Library
  • Molecular Sequence Data
  • Escherichia coli
  • Elastin
  • Cations