Skip to main content
Journal cover image

Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution.

Publication ,  Journal Article
Lim, DW; Nettles, DL; Setton, LA; Chilkoti, A
Published in: Biomacromolecules
May 2007

In situ gelation of injectable polypeptide-based materials is attractive for minimally invasive in vivo implantation of biomaterials and tissue engineering scaffolds. We demonstrate that chemically cross-linked elastin-like polypeptide (ELP) hydrogels can be rapidly formed in aqueous solution by reacting lysine-containing ELPs with an organophosphorous cross-linker, beta-[tris(hydroxymethyl)phosphino]propionic acid (THPP) under physiological conditions. The mechanical properties of the cross-linked ELP hydrogels were largely modulated by the molar concentration of lysine residues in the ELP and the pH at which the cross-linking reaction was carried out. Fibroblasts embedded in ELP hydrogels survived the cross-linking process and were viable after in vitro culture for 3 days. DNA quantification of ELP hydrogels with encapsulated fibroblasts indicated that there was no significant difference in DNA content between day 0 and day 3 when ELP hydrogels were formed with an equimolar ratio of THPP and lysine residues of the ELPs. These results suggest that THPP cross-linking may be a biocompatible strategy for the in situ formation of cross-linked hydrogels.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

May 2007

Volume

8

Issue

5

Start / End Page

1463 / 1470

Related Subject Headings

  • Water
  • Solutions
  • Propionates
  • Polymers
  • Phosphines
  • Peptides
  • Peptide Library
  • Organophosphorus Compounds
  • NIH 3T3 Cells
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lim, D. W., Nettles, D. L., Setton, L. A., & Chilkoti, A. (2007). Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution. Biomacromolecules, 8(5), 1463–1470. https://doi.org/10.1021/bm061059m
Lim, Dong Woo, Dana L. Nettles, Lori A. Setton, and Ashutosh Chilkoti. “Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution.Biomacromolecules 8, no. 5 (May 2007): 1463–70. https://doi.org/10.1021/bm061059m.
Lim DW, Nettles DL, Setton LA, Chilkoti A. Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution. Biomacromolecules. 2007 May;8(5):1463–70.
Lim, Dong Woo, et al. “Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution.Biomacromolecules, vol. 8, no. 5, May 2007, pp. 1463–70. Epmc, doi:10.1021/bm061059m.
Lim DW, Nettles DL, Setton LA, Chilkoti A. Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution. Biomacromolecules. 2007 May;8(5):1463–1470.
Journal cover image

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

May 2007

Volume

8

Issue

5

Start / End Page

1463 / 1470

Related Subject Headings

  • Water
  • Solutions
  • Propionates
  • Polymers
  • Phosphines
  • Peptides
  • Peptide Library
  • Organophosphorus Compounds
  • NIH 3T3 Cells
  • Mice