Skip to main content

Label-free measurement of microbicidal gel thickness using low-coherence interferometry.

Publication ,  Journal Article
Braun, KE; Boyer, JD; Henderson, MH; Katz, DF; Wax, A
Published in: Journal of biomedical optics
March 2006

Spectral-domain low-coherence interferometry (LCI) was used to measure the thickness of microbicidal gels applied to a cylindrical calibration test socket. Microbicides are topical formulations containing active ingredients targeted to inhibit specific pathogens that are currently under development for application to the epithelial lining of the lower female reproductive tract to combat sexually transmitted infections such as HIV. Understanding the deployment and drug delivery of these formulations is vital to maximizing their effectiveness. Previously, in vivo measurements of microbicidal formulation thickness were assessed using fluorescence measurements of fluorescein-labeled gels via an optical endoscope-based device. Here we present an LCI-based device that measures the thickness of a formulation without the use of any exogenous agents by analyzing the interference pattern generated between the reflections from the front and back surface of the sample. Results are presented that validate the effectiveness and performance of the LCI measurement in a clinically relevant system as compared to an existing fluorescence-based method. The impact of the new LCI-based design on in vivo measurements is discussed.

Duke Scholars

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

March 2006

Volume

11

Issue

2

Start / End Page

020504

Related Subject Headings

  • Tomography, Optical Coherence
  • Staining and Labeling
  • Optics
  • Materials Testing
  • Interferometry
  • Gels
  • Drug Evaluation
  • Anti-Infective Agents
  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Braun, K. E., Boyer, J. D., Henderson, M. H., Katz, D. F., & Wax, A. (2006). Label-free measurement of microbicidal gel thickness using low-coherence interferometry. Journal of Biomedical Optics, 11(2), 020504. https://doi.org/10.1117/1.2192767
Braun, Kelly E., Jeffrey D. Boyer, Marcus H. Henderson, David F. Katz, and Adam Wax. “Label-free measurement of microbicidal gel thickness using low-coherence interferometry.Journal of Biomedical Optics 11, no. 2 (March 2006): 020504. https://doi.org/10.1117/1.2192767.
Braun KE, Boyer JD, Henderson MH, Katz DF, Wax A. Label-free measurement of microbicidal gel thickness using low-coherence interferometry. Journal of biomedical optics. 2006 Mar;11(2):020504.
Braun, Kelly E., et al. “Label-free measurement of microbicidal gel thickness using low-coherence interferometry.Journal of Biomedical Optics, vol. 11, no. 2, Mar. 2006, p. 020504. Epmc, doi:10.1117/1.2192767.
Braun KE, Boyer JD, Henderson MH, Katz DF, Wax A. Label-free measurement of microbicidal gel thickness using low-coherence interferometry. Journal of biomedical optics. 2006 Mar;11(2):020504.

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

March 2006

Volume

11

Issue

2

Start / End Page

020504

Related Subject Headings

  • Tomography, Optical Coherence
  • Staining and Labeling
  • Optics
  • Materials Testing
  • Interferometry
  • Gels
  • Drug Evaluation
  • Anti-Infective Agents
  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering