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Circadian clock-controlled genes isolated from Neurospora crassa are late night- to early morning-specific.

Publication ,  Journal Article
Bell-Pedersen, D; Shinohara, ML; Loros, JJ; Dunlap, JC
Published in: Proc Natl Acad Sci U S A
November 12, 1996

An endogenous circadian biological clock controls the temporal aspects of life in most organisms, including rhythmic control of genes involved in clock output pathways. In the fungus Neurospora crassa, one pathway known to be under control of the clock is asexual spore (conidia) development. To understand more fully the processes that are regulated by the N. crassa circadian clock, systematic screens were carried out for genes that oscillate at the transcriptional level. Time-of-day-specific cDNA libraries were generated and used in differential screens to identify six new clock-controlled genes (ccgs). Transcripts specific for each of the ccgs preferentially accumulate during the late night to early morning, although they vary with respect to steady-state mRNA levels and amplitude of the rhythm. Sequencing of the ends of the new ccg cDNAs revealed that ccg-12 is identical to N. crassa cmt encoding copper metallothionein, providing the suggestion that not all clock-regulated genes in N. crassa are specifically involved in the development of conidia. This was supported by finding that half of the new ccgs, including cmt(ccg-12), are not transcriptionally induced by developmental or light signals. These data suggest a major role for the clock in the regulation of biological processes distinct from development.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 12, 1996

Volume

93

Issue

23

Start / End Page

13096 / 13101

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Spores, Fungal
  • RNA, Messenger
  • RNA, Fungal
  • Polymorphism, Restriction Fragment Length
  • Plasmids
  • Neurospora crassa
  • Genome, Fungal
  • Genetic Linkage
  • Gene Library
 

Citation

APA
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MLA
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Bell-Pedersen, D., Shinohara, M. L., Loros, J. J., & Dunlap, J. C. (1996). Circadian clock-controlled genes isolated from Neurospora crassa are late night- to early morning-specific. Proc Natl Acad Sci U S A, 93(23), 13096–13101. https://doi.org/10.1073/pnas.93.23.13096
Bell-Pedersen, D., M. L. Shinohara, J. J. Loros, and J. C. Dunlap. “Circadian clock-controlled genes isolated from Neurospora crassa are late night- to early morning-specific.Proc Natl Acad Sci U S A 93, no. 23 (November 12, 1996): 13096–101. https://doi.org/10.1073/pnas.93.23.13096.
Bell-Pedersen D, Shinohara ML, Loros JJ, Dunlap JC. Circadian clock-controlled genes isolated from Neurospora crassa are late night- to early morning-specific. Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13096–101.
Bell-Pedersen, D., et al. “Circadian clock-controlled genes isolated from Neurospora crassa are late night- to early morning-specific.Proc Natl Acad Sci U S A, vol. 93, no. 23, Nov. 1996, pp. 13096–101. Pubmed, doi:10.1073/pnas.93.23.13096.
Bell-Pedersen D, Shinohara ML, Loros JJ, Dunlap JC. Circadian clock-controlled genes isolated from Neurospora crassa are late night- to early morning-specific. Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13096–13101.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 12, 1996

Volume

93

Issue

23

Start / End Page

13096 / 13101

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Spores, Fungal
  • RNA, Messenger
  • RNA, Fungal
  • Polymorphism, Restriction Fragment Length
  • Plasmids
  • Neurospora crassa
  • Genome, Fungal
  • Genetic Linkage
  • Gene Library