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Imaging and measurement of cell structure and organization with submicron accuracy using light scattering spectroscopy

Publication ,  Journal Article
Backman, V; Gurjar, R; Perelman, LT; Gopal, V; Kalashnikov, M; Badizadegan, K; Wax, A; Georgakoudi, I; Mueller, M; Boone, CW; Itzkan, I ...
Published in: Proceedings of SPIE-The International Society for Optical Engineering
January 1, 2002

Light scattering spectroscopy (LSS) is a promising optical technique developed for quantitative characterization of tissue morphology as well as in vivo detection and diagnosis of disease such as early cancer. LSS employs a wavelength dependent component of light scattered by epithelial cells and other tissues to obtain information about subcellular structure. We present two novel modalities of LSS, LSS imaging and scattering angle sensitive LSS (a/LSS). LSS imaging provides quantitative information about the epithelial cell nuclei, such as nuclear size, degree of pleomorphism, hyperchromasia, and amount of chromatin. It allows mapping these histological properties over wide areas of epithelial lining. We show that LSS imaging can be used to detect precancerous lesions in optically accessible organs. Using a/LSS, which enables characterization of tissue components with sizes smaller than the wavelength of light, we show that the number of subcellular components with the sizes between 30 nm and few microns scales with the size according to an inverse power-law. We show that the size distribution exponent is an important parameter characterizing tissue organization, for example the balance between stochasticity and order, and has a potential to be applicable for early cancer diagnosis and characterization.

Duke Scholars

Published In

Proceedings of SPIE-The International Society for Optical Engineering

DOI

ISSN

0277-786X

Publication Date

January 1, 2002

Volume

4613

Start / End Page

101 / 110

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

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Backman, V., Gurjar, R., Perelman, L. T., Gopal, V., Kalashnikov, M., Badizadegan, K., … Feld, M. S. (2002). Imaging and measurement of cell structure and organization with submicron accuracy using light scattering spectroscopy. Proceedings of SPIE-The International Society for Optical Engineering, 4613, 101–110. https://doi.org/10.1117/12.465234
Backman, V., R. Gurjar, L. T. Perelman, V. Gopal, M. Kalashnikov, K. Badizadegan, A. Wax, et al. “Imaging and measurement of cell structure and organization with submicron accuracy using light scattering spectroscopy.” Proceedings of SPIE-The International Society for Optical Engineering 4613 (January 1, 2002): 101–10. https://doi.org/10.1117/12.465234.
Backman V, Gurjar R, Perelman LT, Gopal V, Kalashnikov M, Badizadegan K, et al. Imaging and measurement of cell structure and organization with submicron accuracy using light scattering spectroscopy. Proceedings of SPIE-The International Society for Optical Engineering. 2002 Jan 1;4613:101–10.
Backman, V., et al. “Imaging and measurement of cell structure and organization with submicron accuracy using light scattering spectroscopy.” Proceedings of SPIE-The International Society for Optical Engineering, vol. 4613, Jan. 2002, pp. 101–10. Scopus, doi:10.1117/12.465234.
Backman V, Gurjar R, Perelman LT, Gopal V, Kalashnikov M, Badizadegan K, Wax A, Georgakoudi I, Mueller M, Boone CW, Itzkan I, Dasari RR, Feld MS. Imaging and measurement of cell structure and organization with submicron accuracy using light scattering spectroscopy. Proceedings of SPIE-The International Society for Optical Engineering. 2002 Jan 1;4613:101–110.

Published In

Proceedings of SPIE-The International Society for Optical Engineering

DOI

ISSN

0277-786X

Publication Date

January 1, 2002

Volume

4613

Start / End Page

101 / 110

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering