Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability.
Publication
, Journal Article
Garg, PK; Slade, SK; Harrison, CL; Zalutsky, MR
Published in: Int J Rad Appl Instrum B
1989
The effect of para vs meta substitution on the biological behavior of an intact antibody and an F(ab')2 fragment was investigated. Paired-label studies were performed using 81C6 IgG and OC 125 F(ab')2 labeled using the N-succinimidyl esters of both p-[125I]- and m-[131I]iodobenzoate as well as with the potential catabolites, p-[125I]- and m-[131I]iodobenzoic acid. In all 3 studies, up to 55% lower uptake of 131I in thyroid and stomach was observed, suggesting that the m-substituted species were more inert to dehalogenation in vivo.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
Int J Rad Appl Instrum B
DOI
ISSN
0883-2897
Publication Date
1989
Volume
16
Issue
7
Start / End Page
669 / 673
Location
England
Related Subject Headings
- Tissue Distribution
- Neoplasm Proteins
- Mice, Inbred BALB C
- Mice
- Male
- Isotope Labeling
- Iodobenzoates
- Iodine Radioisotopes
- Immunoglobulin Fab Fragments
- Drug Stability
Citation
APA
Chicago
ICMJE
MLA
NLM
Garg, P. K., Slade, S. K., Harrison, C. L., & Zalutsky, M. R. (1989). Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability. Int J Rad Appl Instrum B, 16(7), 669–673. https://doi.org/10.1016/0883-2897(89)90136-0
Garg, P. K., S. K. Slade, C. L. Harrison, and M. R. Zalutsky. “Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability.” Int J Rad Appl Instrum B 16, no. 7 (1989): 669–73. https://doi.org/10.1016/0883-2897(89)90136-0.
Garg PK, Slade SK, Harrison CL, Zalutsky MR. Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability. Int J Rad Appl Instrum B. 1989;16(7):669–73.
Garg, P. K., et al. “Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability.” Int J Rad Appl Instrum B, vol. 16, no. 7, 1989, pp. 669–73. Pubmed, doi:10.1016/0883-2897(89)90136-0.
Garg PK, Slade SK, Harrison CL, Zalutsky MR. Labeling proteins using aryl iodide acylation agents: influence of meta vs para substitution on in vivo stability. Int J Rad Appl Instrum B. 1989;16(7):669–673.
Published In
Int J Rad Appl Instrum B
DOI
ISSN
0883-2897
Publication Date
1989
Volume
16
Issue
7
Start / End Page
669 / 673
Location
England
Related Subject Headings
- Tissue Distribution
- Neoplasm Proteins
- Mice, Inbred BALB C
- Mice
- Male
- Isotope Labeling
- Iodobenzoates
- Iodine Radioisotopes
- Immunoglobulin Fab Fragments
- Drug Stability