Skip to main content
Journal cover image

Peroxynitrite flux-mediated LDL oxidation is inhibited by manganese porphyrins in the presence of uric acid.

Publication ,  Journal Article
Trostchansky, A; Ferrer-Sueta, G; Batthyány, C; Botti, H; Batinić-Haberle, I; Radi, R; Rubbo, H
Published in: Free Radic Biol Med
November 15, 2003

We have studied the role of three Mn(III)porphyrins differing in charge, alkyl substituent length and reactivity, on LDL exposed to low fluxes of peroxynitrite (PN) in the presence of uric acid. Mn(III)porphyrins (5 microM, MnTE-2-PyP(5+), MnTnOct-2-PyP(5+), and MnTCPP(3-)) plus uric acid (300 microM) inhibited cholesteryl ester hydroperoxide formation, changes in REM as well as spared alpha- and gamma-tocopherol. MnTnOct-2-PyP(5+), the more lipophilic compound, was the most effective in protecting LDL lipids, while MnTCPP(3-) exerted the lesser protection. Mn(III)porphyrins react fast with PN ( approximately 10(5)-10(7) M(-1) s(-1)) to yield a O=Mn(IV) complex. The stoichiometry of uric acid consumption was approximately 1.7 moles per mol of PN, in agreement with reactions with both the O=Mn(IV) complex and nitrogen dioxide. A shift from an anti- to a pro-oxidant action of the Mn(III)porphyrin was observed after uric acid was significantly consumed, supporting competition reactions between LDL targets and uric acid for the O=Mn(IV) complex. Overall, the data is consistent with the catalytic reduction of PN in a cycle that involves a one electron oxidation of Mn(III) to Mn(IV) by PN followed by the reduction back to Mn(III) by uric acid. These antioxidant effects should predominate under in vivo conditions having plasma uric acid concentration range between 150 and 500 microM.

Duke Scholars

Published In

Free Radic Biol Med

DOI

ISSN

0891-5849

Publication Date

November 15, 2003

Volume

35

Issue

10

Start / End Page

1293 / 1300

Location

United States

Related Subject Headings

  • gamma-Tocopherol
  • alpha-Tocopherol
  • Uric Acid
  • Peroxynitrous Acid
  • Oxidation-Reduction
  • Nitrogen Dioxide
  • Metalloporphyrins
  • Manganese
  • Lipoproteins, LDL
  • Hydrogen Peroxide
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Trostchansky, A., Ferrer-Sueta, G., Batthyány, C., Botti, H., Batinić-Haberle, I., Radi, R., & Rubbo, H. (2003). Peroxynitrite flux-mediated LDL oxidation is inhibited by manganese porphyrins in the presence of uric acid. Free Radic Biol Med, 35(10), 1293–1300. https://doi.org/10.1016/j.freeradbiomed.2003.07.004
Trostchansky, Andrés, Gerardo Ferrer-Sueta, Carlos Batthyány, Horacio Botti, Ines Batinić-Haberle, Rafael Radi, and Homero Rubbo. “Peroxynitrite flux-mediated LDL oxidation is inhibited by manganese porphyrins in the presence of uric acid.Free Radic Biol Med 35, no. 10 (November 15, 2003): 1293–1300. https://doi.org/10.1016/j.freeradbiomed.2003.07.004.
Trostchansky A, Ferrer-Sueta G, Batthyány C, Botti H, Batinić-Haberle I, Radi R, et al. Peroxynitrite flux-mediated LDL oxidation is inhibited by manganese porphyrins in the presence of uric acid. Free Radic Biol Med. 2003 Nov 15;35(10):1293–300.
Trostchansky, Andrés, et al. “Peroxynitrite flux-mediated LDL oxidation is inhibited by manganese porphyrins in the presence of uric acid.Free Radic Biol Med, vol. 35, no. 10, Nov. 2003, pp. 1293–300. Pubmed, doi:10.1016/j.freeradbiomed.2003.07.004.
Trostchansky A, Ferrer-Sueta G, Batthyány C, Botti H, Batinić-Haberle I, Radi R, Rubbo H. Peroxynitrite flux-mediated LDL oxidation is inhibited by manganese porphyrins in the presence of uric acid. Free Radic Biol Med. 2003 Nov 15;35(10):1293–1300.
Journal cover image

Published In

Free Radic Biol Med

DOI

ISSN

0891-5849

Publication Date

November 15, 2003

Volume

35

Issue

10

Start / End Page

1293 / 1300

Location

United States

Related Subject Headings

  • gamma-Tocopherol
  • alpha-Tocopherol
  • Uric Acid
  • Peroxynitrous Acid
  • Oxidation-Reduction
  • Nitrogen Dioxide
  • Metalloporphyrins
  • Manganese
  • Lipoproteins, LDL
  • Hydrogen Peroxide