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A computer model of engineered cardiac monolayers.

Publication ,  Journal Article
Kim, JM; Bursac, N; Henriquez, CS
Published in: Biophysical journal
May 2010

Engineered monolayers created using microabrasion and micropatterning methods have provided a simplified in vitro system to study the effects of anisotropy and fiber direction on electrical propagation. Interpreting the behavior in these culture systems has often been performed using classical computer models with continuous properties. However, such models do not account for the effects of random cell shapes, cell orientations, and cleft spaces inherent in these monolayers on the resulting wavefront conduction. This work presents a novel methodology for modeling a monolayer of cardiac tissue in which the factors governing cell shape, cell-to-cell coupling, and degree of cleft space are not constant but rather are treated as spatially random with assigned distributions. This modeling approach makes it possible to simulate wavefront propagation in a manner analogous to performing experiments on engineered monolayer tissues. Simulated results are compared to previously published measured data from monolayers used to investigate the role of cellular architecture on conduction velocities and anisotropy ratios. We also present an estimate for obtaining the electrical properties from these networks and demonstrate how variations in the discrete cellular architecture affect the macroscopic conductivities. The simulations support the common assumption that under normal ranges of coupling strength, tissues with relatively uniform distributions of cell shapes and connectivity can be represented using continuous models with conductivities derived from random discrete cellular architecture using either global or local estimates. The results also reveal that in the presence of abrupt changes in cell orientation, local estimates of tissue properties predict smoother changes in conductivity that may not adequately predict the discrete nature of propagation at the transition sites.

Duke Scholars

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

May 2010

Volume

98

Issue

9

Start / End Page

1762 / 1771

Related Subject Headings

  • Tissue Engineering
  • Rats
  • Myocardium
  • Models, Biological
  • Mice
  • Kinetics
  • Intracellular Space
  • Electric Conductivity
  • Computer Simulation
  • Cell Shape
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kim, J. M., Bursac, N., & Henriquez, C. S. (2010). A computer model of engineered cardiac monolayers. Biophysical Journal, 98(9), 1762–1771. https://doi.org/10.1016/j.bpj.2010.01.008
Kim, Jong M., Nenad Bursac, and Craig S. Henriquez. “A computer model of engineered cardiac monolayers.Biophysical Journal 98, no. 9 (May 2010): 1762–71. https://doi.org/10.1016/j.bpj.2010.01.008.
Kim JM, Bursac N, Henriquez CS. A computer model of engineered cardiac monolayers. Biophysical journal. 2010 May;98(9):1762–71.
Kim, Jong M., et al. “A computer model of engineered cardiac monolayers.Biophysical Journal, vol. 98, no. 9, May 2010, pp. 1762–71. Epmc, doi:10.1016/j.bpj.2010.01.008.
Kim JM, Bursac N, Henriquez CS. A computer model of engineered cardiac monolayers. Biophysical journal. 2010 May;98(9):1762–1771.
Journal cover image

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

May 2010

Volume

98

Issue

9

Start / End Page

1762 / 1771

Related Subject Headings

  • Tissue Engineering
  • Rats
  • Myocardium
  • Models, Biological
  • Mice
  • Kinetics
  • Intracellular Space
  • Electric Conductivity
  • Computer Simulation
  • Cell Shape