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Quantitative model of the phase behavior of recombinant pH-responsive elastin-like polypeptides.

Publication ,  Journal Article
Mackay, JA; Callahan, DJ; Fitzgerald, KN; Chilkoti, A
Published in: Biomacromolecules
November 2010

Quantitative models are required to engineer biomaterials with environmentally responsive properties. With this goal in mind, we developed a model that describes the pH-dependent phase behavior of a class of stimulus responsive elastin-like polypeptides (ELPs) that undergo reversible phase separation in response to their solution environment. Under isothermal conditions, charged ELPs can undergo phase separation when their charge is neutralized. Optimization of this behavior has been challenging because the pH at which they phase separate, pHt, depends on their composition, molecular weight, concentration, and temperature. To address this problem, we developed a quantitative model to describe the phase behavior of charged ELPs that uses the Henderson-Hasselbalch relationship to describe the effect of side-chain ionization on the phase-transition temperature of an ELP. The model was validated with pH-responsive ELPs that contained either acidic (Glu) or basic (His) residues. The phase separation of both ELPs fit this model across a range of pH. These results have important implications for applications of pH-responsive ELPs because they provide a quantitative model for the rational design of pH-responsive polypeptides whose transition can be triggered at a specified pH.

Duke Scholars

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

November 2010

Volume

11

Issue

11

Start / End Page

2873 / 2879

Related Subject Headings

  • Temperature
  • Polymers
  • Phase Transition
  • Peptides
  • Models, Molecular
  • Hydrogen-Ion Concentration
  • Elastin
  • 40 Engineering
  • 34 Chemical sciences
  • 31 Biological sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Mackay, J. A., Callahan, D. J., Fitzgerald, K. N., & Chilkoti, A. (2010). Quantitative model of the phase behavior of recombinant pH-responsive elastin-like polypeptides. Biomacromolecules, 11(11), 2873–2879. https://doi.org/10.1021/bm100571j
Mackay, J Andrew, Daniel J. Callahan, Kelly N. Fitzgerald, and Ashutosh Chilkoti. “Quantitative model of the phase behavior of recombinant pH-responsive elastin-like polypeptides.Biomacromolecules 11, no. 11 (November 2010): 2873–79. https://doi.org/10.1021/bm100571j.
Mackay JA, Callahan DJ, Fitzgerald KN, Chilkoti A. Quantitative model of the phase behavior of recombinant pH-responsive elastin-like polypeptides. Biomacromolecules. 2010 Nov;11(11):2873–9.
Mackay, J. Andrew, et al. “Quantitative model of the phase behavior of recombinant pH-responsive elastin-like polypeptides.Biomacromolecules, vol. 11, no. 11, Nov. 2010, pp. 2873–79. Epmc, doi:10.1021/bm100571j.
Mackay JA, Callahan DJ, Fitzgerald KN, Chilkoti A. Quantitative model of the phase behavior of recombinant pH-responsive elastin-like polypeptides. Biomacromolecules. 2010 Nov;11(11):2873–2879.
Journal cover image

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

November 2010

Volume

11

Issue

11

Start / End Page

2873 / 2879

Related Subject Headings

  • Temperature
  • Polymers
  • Phase Transition
  • Peptides
  • Models, Molecular
  • Hydrogen-Ion Concentration
  • Elastin
  • 40 Engineering
  • 34 Chemical sciences
  • 31 Biological sciences