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Elastin-like polypeptides as a purification tag for recombinant proteins.

Publication ,  Journal Article
Hassouneh, W; Christensen, T; Chilkoti, A
Published in: Current protocols in protein science
August 2010

This unit presents a recombinant protein purification method that employs an elastin-like polypeptide (ELP) as a purification tag. ELPs undergo a sharp and reversible phase transition when heated above their lower critical solution temperature. ELPs retain this behavior when they are fused to a protein, and thereby provide a simple method to isolate a recombinant ELP fusion protein from cell contaminants by cycling the solution through the insoluble and soluble phase of the ELP fusion protein using a procedure that is termed Inverse Transition Cycling. This method does not require the use of chromatography, so it is cost-effective, easy to scale up, and easy to multiplex.

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Published In

Current protocols in protein science

DOI

EISSN

1934-3663

ISSN

1934-3655

Publication Date

August 2010

Volume

Chapter 6

Start / End Page

6.11.1 / 6.11.16

Related Subject Headings

  • Temperature
  • Recombinant Fusion Proteins
  • Peptides
  • Particle Size
  • Escherichia coli
  • Elastin
  • Chromatography, Affinity
 

Citation

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Hassouneh, W., Christensen, T., & Chilkoti, A. (2010). Elastin-like polypeptides as a purification tag for recombinant proteins. Current Protocols in Protein Science, Chapter 6, 6.11.1-6.11.16. https://doi.org/10.1002/0471140864.ps0611s61
Hassouneh, Wafa, Trine Christensen, and Ashutosh Chilkoti. “Elastin-like polypeptides as a purification tag for recombinant proteins.Current Protocols in Protein Science Chapter 6 (August 2010): 6.11.1-6.11.16. https://doi.org/10.1002/0471140864.ps0611s61.
Hassouneh W, Christensen T, Chilkoti A. Elastin-like polypeptides as a purification tag for recombinant proteins. Current protocols in protein science. 2010 Aug;Chapter 6:6.11.1-6.11.16.
Hassouneh, Wafa, et al. “Elastin-like polypeptides as a purification tag for recombinant proteins.Current Protocols in Protein Science, vol. Chapter 6, Aug. 2010, pp. 6.11.1-6.11.16. Epmc, doi:10.1002/0471140864.ps0611s61.
Hassouneh W, Christensen T, Chilkoti A. Elastin-like polypeptides as a purification tag for recombinant proteins. Current protocols in protein science. 2010 Aug;Chapter 6:6.11.1-6.11.16.

Published In

Current protocols in protein science

DOI

EISSN

1934-3663

ISSN

1934-3655

Publication Date

August 2010

Volume

Chapter 6

Start / End Page

6.11.1 / 6.11.16

Related Subject Headings

  • Temperature
  • Recombinant Fusion Proteins
  • Peptides
  • Particle Size
  • Escherichia coli
  • Elastin
  • Chromatography, Affinity