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A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineering

Publication ,  Journal Article
Betre, H; Chilkoti, A; Setton, LA
Published in: Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
December 1, 2002

A "two step" tissue engineering strategy was developed to promote rapid matrix accumulation in cartilage constructs in vitro. Chondrocytes expanded in monolayer were encapsulated and cultured in a genetically engineered, thermally sensitive elastin-like polypeptide (ELP) for ten days. The resulting cell-matrix pellets were recovered from the ELP and cultured on inserts for up to four weeks, where nutrient diffusion was not impeded by the presence of the scaffold. Approximately two-milligram (dry weight) tissue was generated that resembles native articular cartilage in histological appearance and biochemical composition. These results suggest that rapid and large cartilage construct formation is possible in vitro, following a period of early incubation and recovery from the thermally responsive ELP.

Duke Scholars

Published In

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

ISSN

0589-1019

Publication Date

December 1, 2002

Volume

1

Start / End Page

829 / 830
 

Citation

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Betre, H., Chilkoti, A., & Setton, L. A. (2002). A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineering. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, 1, 829–830.
Betre, H., A. Chilkoti, and L. A. Setton. “A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineering.” Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings 1 (December 1, 2002): 829–30.
Betre H, Chilkoti A, Setton LA. A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineering. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2002 Dec 1;1:829–30.
Betre, H., et al. “A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineering.” Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, vol. 1, Dec. 2002, pp. 829–30.
Betre H, Chilkoti A, Setton LA. A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineering. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2002 Dec 1;1:829–830.

Published In

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

ISSN

0589-1019

Publication Date

December 1, 2002

Volume

1

Start / End Page

829 / 830