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Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein.

Publication ,  Journal Article
Chilkoti, A; Chen, G; Stayton, PS; Hoffman, AS
Published in: Bioconjugate chemistry
November 1994

A genetically-engineered mutant of cytochrome b5, incorporating a unique cysteine residue, was conjugated to maleimide-terminated oligo(N-isopropylacrylamide). The conjugation of the protein by reaction of the cysteine residue, precisely positioned by site-directed mutagenesis techniques, with an activated oligomer containing only one reactive end group in the oligomer chain permits the site-specific and stoichiometric conjugation of the oligomer with the protein. The protein-oligomer conjugate was shown to exhibit lower critical solution temperature (LCST) behavior, similar to the free oligomer. Furthermore, the LCST behavior of the protein-oligomer conjugate is reversible and allows selective precipitation of the conjugate above its LCST.

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Published In

Bioconjugate chemistry

DOI

EISSN

1520-4812

ISSN

1043-1802

Publication Date

November 1994

Volume

5

Issue

6

Start / End Page

504 / 507

Related Subject Headings

  • Temperature
  • Protein Engineering
  • Polymers
  • Organic Chemistry
  • Mutagenesis, Site-Directed
  • Mass Spectrometry
  • Maleimides
  • Cytochromes b5
  • Cysteine
  • Binding Sites
 

Citation

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Chilkoti, A., Chen, G., Stayton, P. S., & Hoffman, A. S. (1994). Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein. Bioconjugate Chemistry, 5(6), 504–507. https://doi.org/10.1021/bc00030a004
Chilkoti, A., G. Chen, P. S. Stayton, and A. S. Hoffman. “Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein.Bioconjugate Chemistry 5, no. 6 (November 1994): 504–7. https://doi.org/10.1021/bc00030a004.
Chilkoti A, Chen G, Stayton PS, Hoffman AS. Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein. Bioconjugate chemistry. 1994 Nov;5(6):504–7.
Chilkoti, A., et al. “Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein.Bioconjugate Chemistry, vol. 5, no. 6, Nov. 1994, pp. 504–07. Epmc, doi:10.1021/bc00030a004.
Chilkoti A, Chen G, Stayton PS, Hoffman AS. Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein. Bioconjugate chemistry. 1994 Nov;5(6):504–507.
Journal cover image

Published In

Bioconjugate chemistry

DOI

EISSN

1520-4812

ISSN

1043-1802

Publication Date

November 1994

Volume

5

Issue

6

Start / End Page

504 / 507

Related Subject Headings

  • Temperature
  • Protein Engineering
  • Polymers
  • Organic Chemistry
  • Mutagenesis, Site-Directed
  • Mass Spectrometry
  • Maleimides
  • Cytochromes b5
  • Cysteine
  • Binding Sites