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Engineered proteins for biomaterials

Publication ,  Journal Article
Stayton, PS; Chilkoti, A; Long, CJ; Pettit, DK; Tan, PHS; Chen, G; Hoffman, AS
Published in: Materials Research Society Symposium Proceedings
January 1, 1993

A molecular adaptor for interfacing environmentally sensitive, soluble polymers and antibody molecules has been developed. The gene coding for the minimally sized, 55 amino acid IgG binding domain from protein G has been constructed by total gene synthesis. This domain is thermally stable, exhibits a highly reversible folding and unfolding equilibrium, and recognizes IgG and Fab molecules with high affinity. These properties make the protein G domain a potentially useful adaptor for non-covalent immobilization of antibodies to soluble polymers and hydrogels. Engineered single-chain Fv antibody fragments have also been constructed and a method for expanding the usefulness of the protein G adaptor to these molecules is proposed. The engineered antibodies also provide a model system for developing general immobilization strategies aimed at maximizing binding affinities and therapeutic responses. The overall goal is to develop optimized engineering designs for functionally optimized antibody-material hybrids.

Duke Scholars

Published In

Materials Research Society Symposium Proceedings

ISSN

0272-9172

Publication Date

January 1, 1993

Volume

292

Start / End Page

77 / 82
 

Citation

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Stayton, P. S., Chilkoti, A., Long, C. J., Pettit, D. K., Tan, P. H. S., Chen, G., & Hoffman, A. S. (1993). Engineered proteins for biomaterials. Materials Research Society Symposium Proceedings, 292, 77–82.
Stayton, P. S., A. Chilkoti, C. J. Long, D. K. Pettit, P. H. S. Tan, G. Chen, and A. S. Hoffman. “Engineered proteins for biomaterials.” Materials Research Society Symposium Proceedings 292 (January 1, 1993): 77–82.
Stayton PS, Chilkoti A, Long CJ, Pettit DK, Tan PHS, Chen G, et al. Engineered proteins for biomaterials. Materials Research Society Symposium Proceedings. 1993 Jan 1;292:77–82.
Stayton, P. S., et al. “Engineered proteins for biomaterials.” Materials Research Society Symposium Proceedings, vol. 292, Jan. 1993, pp. 77–82.
Stayton PS, Chilkoti A, Long CJ, Pettit DK, Tan PHS, Chen G, Hoffman AS. Engineered proteins for biomaterials. Materials Research Society Symposium Proceedings. 1993 Jan 1;292:77–82.

Published In

Materials Research Society Symposium Proceedings

ISSN

0272-9172

Publication Date

January 1, 1993

Volume

292

Start / End Page

77 / 82