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Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors.

Publication ,  Journal Article
Mukoyama, M; Nakajima, M; Horiuchi, M; Sasamura, H; Pratt, RE; Dzau, VJ
Published in: J Biol Chem
November 25, 1993

Angiotensin II acts on at least two distinct receptor subtypes (AT1 and AT2). Most known effects of angiotensin II in adult tissues are attributable to the AT1 receptor. The function of AT2 receptor is undefined, but its abundant expressions in fetal tissues, immature brain, skin wound, and atretic ovarian follicles suggest a role in growth and development. Previous studies suggested that AT2 receptor may not be G protein-coupled. Here, from a rat fetus expression library, we cloned a cDNA encoding a unique 363-amino acid protein with pharmacological specificity, tissue distribution, and developmental pattern of the AT2 receptor. It is 34% identical in sequence to the AT1 receptor, sharing a seven-transmembrane domain topology. A review of prior data on other receptors suggests that this receptor may belong to a unique class of seven-transmembrane receptors (including somatostatin SSTR1, dopamine D3, and frizzled protein Fz) for which G protein coupling has not been demonstrated. All members of this class exhibit fetal and developmental and/or neuronal-specific expression. A conserved motif in the third intracellular loop, distinguishing this class from "classical" G protein-coupled receptors, may mediate novel intracellular effects.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

November 25, 1993

Volume

268

Issue

33

Start / End Page

24539 / 24542

Location

United States

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Receptors, Angiotensin
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Molecular Sequence Data
  • GTP-Binding Proteins
  • DNA, Complementary
  • Cloning, Molecular
  • Cell Membrane
 

Citation

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Mukoyama, M., Nakajima, M., Horiuchi, M., Sasamura, H., Pratt, R. E., & Dzau, V. J. (1993). Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors. J Biol Chem, 268(33), 24539–24542.
Mukoyama, M., M. Nakajima, M. Horiuchi, H. Sasamura, R. E. Pratt, and V. J. Dzau. “Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors.J Biol Chem 268, no. 33 (November 25, 1993): 24539–42.
Mukoyama M, Nakajima M, Horiuchi M, Sasamura H, Pratt RE, Dzau VJ. Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors. J Biol Chem. 1993 Nov 25;268(33):24539–42.
Mukoyama, M., et al. “Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors.J Biol Chem, vol. 268, no. 33, Nov. 1993, pp. 24539–42.
Mukoyama M, Nakajima M, Horiuchi M, Sasamura H, Pratt RE, Dzau VJ. Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors. J Biol Chem. 1993 Nov 25;268(33):24539–24542.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

November 25, 1993

Volume

268

Issue

33

Start / End Page

24539 / 24542

Location

United States

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Receptors, Angiotensin
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Molecular Sequence Data
  • GTP-Binding Proteins
  • DNA, Complementary
  • Cloning, Molecular
  • Cell Membrane