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Tissue engraftment of hypoxic-preconditioned adipose-derived stem cells improves flap viability.

Publication ,  Journal Article
Hollenbeck, ST; Senghaas, A; Komatsu, I; Zhang, Y; Erdmann, D; Klitzman, B
Published in: Wound Repair Regen
2012

Adipose-derived stem cells (ASCs) have the ability to release multiple growth factors in response to hypoxia. In this study, we investigated the potential of ASCs to prevent tissue ischemia. We found conditioned media from hypoxic ASCs had increased levels of vascular endothelial growth factor (VEGF) and enhanced endothelial cell tubule formation. To investigate the effect of injecting rat ASCs into ischemic flaps, 21 Lewis rats were divided into three groups: control, normal oxygen ASCs (10(6) cells), and hypoxic preconditioned ASCs (10(6) cells). At the time of flap elevation, the distal third of the flap was injected with the treatment group. At 7 days post flap elevation, flap viability was significantly improved with injection of hypoxic preconditioned ASCs. Cluster of differentiation-31-positive cells were more abundant along the margins of flaps injected with ASCs. Fluorescent labeled ASCs localized aside blood vessels or throughout the tissue, dependent on oxygen preconditioning status. Next, we evaluated the effect of hypoxic preconditioning on ASC migration and chemotaxis. Hypoxia did not affect ASC migration on scratch assay or chemotaxis to collagen and laminin. Thus, hypoxic preconditioning of injected ASCs improves flap viability likely through the effects of VEGF release. These effects are modest and represent the limitations of cellular and growth factor-induced angiogenesis in the acute setting of ischemia.

Duke Scholars

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Published In

Wound Repair Regen

DOI

EISSN

1524-475X

Publication Date

2012

Volume

20

Issue

6

Start / End Page

872 / 878

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Surgical Flaps
  • Stem Cells
  • Stem Cell Transplantation
  • Signal Transduction
  • Rats, Inbred Lew
  • Rats
  • Neovascularization, Physiologic
  • Male
  • Ischemia
 

Citation

APA
Chicago
ICMJE
MLA
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Hollenbeck, S. T., Senghaas, A., Komatsu, I., Zhang, Y., Erdmann, D., & Klitzman, B. (2012). Tissue engraftment of hypoxic-preconditioned adipose-derived stem cells improves flap viability. Wound Repair Regen, 20(6), 872–878. https://doi.org/10.1111/j.1524-475X.2012.00854.x
Hollenbeck, Scott T., Annika Senghaas, Issei Komatsu, Ying Zhang, Detlev Erdmann, and Bruce Klitzman. “Tissue engraftment of hypoxic-preconditioned adipose-derived stem cells improves flap viability.Wound Repair Regen 20, no. 6 (2012): 872–78. https://doi.org/10.1111/j.1524-475X.2012.00854.x.
Hollenbeck ST, Senghaas A, Komatsu I, Zhang Y, Erdmann D, Klitzman B. Tissue engraftment of hypoxic-preconditioned adipose-derived stem cells improves flap viability. Wound Repair Regen. 2012;20(6):872–8.
Hollenbeck, Scott T., et al. “Tissue engraftment of hypoxic-preconditioned adipose-derived stem cells improves flap viability.Wound Repair Regen, vol. 20, no. 6, 2012, pp. 872–78. Pubmed, doi:10.1111/j.1524-475X.2012.00854.x.
Hollenbeck ST, Senghaas A, Komatsu I, Zhang Y, Erdmann D, Klitzman B. Tissue engraftment of hypoxic-preconditioned adipose-derived stem cells improves flap viability. Wound Repair Regen. 2012;20(6):872–878.
Journal cover image

Published In

Wound Repair Regen

DOI

EISSN

1524-475X

Publication Date

2012

Volume

20

Issue

6

Start / End Page

872 / 878

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Surgical Flaps
  • Stem Cells
  • Stem Cell Transplantation
  • Signal Transduction
  • Rats, Inbred Lew
  • Rats
  • Neovascularization, Physiologic
  • Male
  • Ischemia