A new H/D exchange- and MALDI-based assay for the quantitative analysis of protein-DNA interactions in solution
Publication
, Journal Article
Ma, L; Powell, KD; Fitzgerald, MC
Published in: Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
December 1, 2002
A new H/D exchange- and MALDI-based assay for the quantitative analysis of protein-DNA interactions in solution was described. The protein and protein-DNA complexes were diluted ten-fold into a series of deuterated exchange buffers differing only in the concentration of the chemical denaturant, urea for SUPREX analysis. The deuterium content of each protein sample was determined after a specified exchange time using MALDI mass spectrometry. It was found that the use of area as a chemical denaturant, as opposed to GdmCl, was critical to the success of SUPREX experiments on protein-DNA binding.
Duke Scholars
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
471 / 472
Citation
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Ma, L., Powell, K. D., & Fitzgerald, M. C. (2002). A new H/D exchange- and MALDI-based assay for the quantitative analysis of protein-DNA interactions in solution. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, 471–472.
Ma, L., K. D. Powell, and M. C. Fitzgerald. “A new H/D exchange- and MALDI-based assay for the quantitative analysis of protein-DNA interactions in solution.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, December 1, 2002, 471–72.
Ma L, Powell KD, Fitzgerald MC. A new H/D exchange- and MALDI-based assay for the quantitative analysis of protein-DNA interactions in solution. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;471–2.
Ma, L., et al. “A new H/D exchange- and MALDI-based assay for the quantitative analysis of protein-DNA interactions in solution.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, Dec. 2002, pp. 471–72.
Ma L, Powell KD, Fitzgerald MC. A new H/D exchange- and MALDI-based assay for the quantitative analysis of protein-DNA interactions in solution. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;471–472.
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
471 / 472