Quantitative protein stability measurement in living bacteria
Publication
, Journal Article
Ghaemmaghami, S; Fitzgerald, MC; Oas, TG
Published in: Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
December 1, 2002
Hydrogen exchange detected by MALDI mass spectrometry was used to measure the thermodynamic stability of proteins. The stability of the N-terminal domain measured in vitro, qualitatively correlated with its degradation because the folding kinetics of the protein was in rapid pre-equilibrium relative to its proteolysis. Some of the hydrogen atoms in peptides and proteins were labile to exchange with the surrounding solvent and a number of these exchangeable hydrogens were protected by the native conformation of the protein. The results show that the measured in vivo stability is 6.7 ± 0.13 kcal/mol and the in vitro stability of the protein obtained by performing H/D exchange on lysed bacteria at pH 8 is 6.7 ± 0.2 kcal/mol.
Duke Scholars
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
109 / 110
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Ghaemmaghami, S., Fitzgerald, M. C., & Oas, T. G. (2002). Quantitative protein stability measurement in living bacteria. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, 109–110.
Ghaemmaghami, S., M. C. Fitzgerald, and T. G. Oas. “Quantitative protein stability measurement in living bacteria.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, December 1, 2002, 109–10.
Ghaemmaghami S, Fitzgerald MC, Oas TG. Quantitative protein stability measurement in living bacteria. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;109–10.
Ghaemmaghami, S., et al. “Quantitative protein stability measurement in living bacteria.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, Dec. 2002, pp. 109–10.
Ghaemmaghami S, Fitzgerald MC, Oas TG. Quantitative protein stability measurement in living bacteria. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;109–110.
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
109 / 110