Novel adenovirus vectors 'capsid-displaying' a human complement inhibitor.
Adenovirus (Ad) vectors are currently the most commonly utilized gene transfer vectors in humans worldwide. Unfortunately, upon contact with the circulatory system, Ads induce several, innate, complement-dependent toxicities that limit the full potential for Ad-based gene transfer applications. Therefore, we have constructed several novel Ad5-based vectors, 'capsid-displaying' as fiber or pIX fusion proteins, a complement-regulatory peptide (COMPinh). These novel Ads dramatically minimize Ad-dependent activation of the human and non-human primate complement systems, as determined by several assays. In summary, our work has shown that a novel COMPinh-displaying Ad5 has the potential for broadening the safe use of Ad vectors in future human applications.
Duke Scholars
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Related Subject Headings
- Transduction, Genetic
- Recombinant Fusion Proteins
- Peptides
- Immunity, Innate
- Humans
- Genetic Vectors
- Complement System Proteins
- Complement Pathway, Alternative
- Complement Inactivating Agents
- Complement Activation
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transduction, Genetic
- Recombinant Fusion Proteins
- Peptides
- Immunity, Innate
- Humans
- Genetic Vectors
- Complement System Proteins
- Complement Pathway, Alternative
- Complement Inactivating Agents
- Complement Activation