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Further investigation of simian immunodeficiency virus Vif function in human cells.

Publication ,  Journal Article
Gaddis, NC; Sheehy, AM; Ahmad, KM; Swanson, CM; Bishop, KN; Beer, BE; Marx, PA; Gao, F; Bibollet-Ruche, F; Hahn, BH; Malim, MH
Published in: J Virol
November 2004

Primate lentivirus Vif proteins function by suppressing the antiviral activity of the cell-encoded apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like (APOBEC) proteins APOBEC3G and APOBEC3F. It has been hypothesized that species-specific susceptibilities of APOBEC proteins to Vif proteins may help govern the transmission of primate lentiviruses to new host species. Consistent with this view and with previous results, we report that the Vif proteins of several diverse simian immunodeficiency viruses (SIVs) that are not known to infect humans are not effective inhibitors of human APOBEC3G or APOBEC3F when assessed in transient-transfection experiments. Unexpectedly, this lack of SIV Vif function did not prevent the replication of two vif-deficient SIVs (SIVtan and SIVmnd1; isolated from tantalus monkeys and mandrills, respectively) in a human T-cell line, HUT78, that expresses both APOBEC 3G and APOBEC3F, a finding which demonstrates that some SIVs are partially resistant to the antiretroviral effects of these enzymes irrespective of Vif function. Additional virus replication studies also revealed that the Vif protein of SIVtan is, in fact, active in human T cells, as it substantially enhanced the replication of its cognate virus and human immunodeficiency virus type 1. In sum, we now consider it improbable that species-specific restrictions to SIV Vif function can explain the lack of human infection with certain SIVs. Instead, our data reveal that the species-specific modulation of Vif function is more complex than previously envisioned and that additional (as-yet-unidentified) viral or host factors may be involved in regulating this dynamic interaction between host and pathogen.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

November 2004

Volume

78

Issue

21

Start / End Page

12041 / 12046

Location

United States

Related Subject Headings

  • vif Gene Products, Human Immunodeficiency Virus
  • Virus Replication
  • Virology
  • Transfection
  • T-Lymphocytes
  • Species Specificity
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Repressor Proteins
  • Proteins
 

Citation

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Gaddis, N. C., Sheehy, A. M., Ahmad, K. M., Swanson, C. M., Bishop, K. N., Beer, B. E., … Malim, M. H. (2004). Further investigation of simian immunodeficiency virus Vif function in human cells. J Virol, 78(21), 12041–12046. https://doi.org/10.1128/JVI.78.21.12041-12046.2004
Gaddis, Nathan C., Ann M. Sheehy, K Muneer Ahmad, Chad M. Swanson, Kate N. Bishop, Brigitte E. Beer, Preston A. Marx, et al. “Further investigation of simian immunodeficiency virus Vif function in human cells.J Virol 78, no. 21 (November 2004): 12041–46. https://doi.org/10.1128/JVI.78.21.12041-12046.2004.
Gaddis NC, Sheehy AM, Ahmad KM, Swanson CM, Bishop KN, Beer BE, et al. Further investigation of simian immunodeficiency virus Vif function in human cells. J Virol. 2004 Nov;78(21):12041–6.
Gaddis, Nathan C., et al. “Further investigation of simian immunodeficiency virus Vif function in human cells.J Virol, vol. 78, no. 21, Nov. 2004, pp. 12041–46. Pubmed, doi:10.1128/JVI.78.21.12041-12046.2004.
Gaddis NC, Sheehy AM, Ahmad KM, Swanson CM, Bishop KN, Beer BE, Marx PA, Gao F, Bibollet-Ruche F, Hahn BH, Malim MH. Further investigation of simian immunodeficiency virus Vif function in human cells. J Virol. 2004 Nov;78(21):12041–12046.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

November 2004

Volume

78

Issue

21

Start / End Page

12041 / 12046

Location

United States

Related Subject Headings

  • vif Gene Products, Human Immunodeficiency Virus
  • Virus Replication
  • Virology
  • Transfection
  • T-Lymphocytes
  • Species Specificity
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Repressor Proteins
  • Proteins