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Hydroxylated ornithine lipids increase stress tolerance in Rhizobium tropici CIAT899.

Publication ,  Journal Article
Vences-Guzmán, MÁ; Guan, Z; Ormeño-Orrillo, E; González-Silva, N; López-Lara, IM; Martínez-Romero, E; Geiger, O; Sohlenkamp, C
Published in: Mol Microbiol
March 2011

Ornithine lipids (OLs) are widespread among Gram-negative bacteria. Their basic structure consists of a 3-hydroxy fatty acyl group attached in amide linkage to the α-amino group of ornithine and a second fatty acyl group ester-linked to the 3-hydroxy position of the first fatty acid. OLs can be hydroxylated within the secondary fatty acyl moiety and this modification has been related to increased stress tolerance. Rhizobium tropici, a nodule-forming α-proteobacterium known for its stress tolerance, forms four different OLs. Studies of the function of these OLs have been hampered due to lack of knowledge about their biosynthesis. Here we describe that OL biosynthesis increases under acid stress and that OLs are enriched in the outer membrane. Using a functional expression screen, the OL hydroxylase OlsE was identified, which in combination with the OL hydroxylase OlsC is responsible for the synthesis of modified OLs in R. tropici. Unlike described OL hydroxylations, the OlsE-catalysed hydroxylation occurs within the ornithine moiety. Mutants deficient in OlsE or OlsC and double mutants deficient in OlsC/OlsE were characterized. R. tropici mutants deficient in OlsC-mediated OL hydroxylation are more susceptible to acid and temperature stress. All three mutants lacking OL hydroxylases are affected during symbiosis.

Duke Scholars

Published In

Mol Microbiol

DOI

EISSN

1365-2958

Publication Date

March 2011

Volume

79

Issue

6

Start / End Page

1496 / 1514

Location

England

Related Subject Headings

  • Stress, Physiological
  • Rhizobium tropici
  • Ornithine
  • Mutation
  • Molecular Structure
  • Mixed Function Oxygenases
  • Microbiology
  • Lipids
  • Hydroxylation
  • Bacterial Proteins
 

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Vences-Guzmán, M. Á., Guan, Z., Ormeño-Orrillo, E., González-Silva, N., López-Lara, I. M., Martínez-Romero, E., … Sohlenkamp, C. (2011). Hydroxylated ornithine lipids increase stress tolerance in Rhizobium tropici CIAT899. Mol Microbiol, 79(6), 1496–1514. https://doi.org/10.1111/j.1365-2958.2011.07535.x
Vences-Guzmán, Miguel Á., Ziqiang Guan, Ernesto Ormeño-Orrillo, Napoleón González-Silva, Isabel M. López-Lara, Esperanza Martínez-Romero, Otto Geiger, and Christian Sohlenkamp. “Hydroxylated ornithine lipids increase stress tolerance in Rhizobium tropici CIAT899.Mol Microbiol 79, no. 6 (March 2011): 1496–1514. https://doi.org/10.1111/j.1365-2958.2011.07535.x.
Vences-Guzmán MÁ, Guan Z, Ormeño-Orrillo E, González-Silva N, López-Lara IM, Martínez-Romero E, et al. Hydroxylated ornithine lipids increase stress tolerance in Rhizobium tropici CIAT899. Mol Microbiol. 2011 Mar;79(6):1496–514.
Vences-Guzmán, Miguel Á., et al. “Hydroxylated ornithine lipids increase stress tolerance in Rhizobium tropici CIAT899.Mol Microbiol, vol. 79, no. 6, Mar. 2011, pp. 1496–514. Pubmed, doi:10.1111/j.1365-2958.2011.07535.x.
Vences-Guzmán MÁ, Guan Z, Ormeño-Orrillo E, González-Silva N, López-Lara IM, Martínez-Romero E, Geiger O, Sohlenkamp C. Hydroxylated ornithine lipids increase stress tolerance in Rhizobium tropici CIAT899. Mol Microbiol. 2011 Mar;79(6):1496–1514.
Journal cover image

Published In

Mol Microbiol

DOI

EISSN

1365-2958

Publication Date

March 2011

Volume

79

Issue

6

Start / End Page

1496 / 1514

Location

England

Related Subject Headings

  • Stress, Physiological
  • Rhizobium tropici
  • Ornithine
  • Mutation
  • Molecular Structure
  • Mixed Function Oxygenases
  • Microbiology
  • Lipids
  • Hydroxylation
  • Bacterial Proteins