Nociceptive-specific activation of ERK in spinal neurons contributes to pain hypersensitivity.
We investigated the involvement of extracellular signal-regulated protein kinases (ERK) within spinal neurons in producing pain hypersensitivity. Within a minute of an intense noxious peripheral or C-fiber electrical stimulus, many phosphoERK-positive neurons were observed, most predominantly in lamina I and IIo of the ipsilateral dorsal horn. This staining was intensity and NMDA receptor dependent. Low-intensity stimuli or A-fiber input had no effect. Inhibition of ERK phosphorylation by a MEK inhibitor reduced the second phase of formalin-induced pain behavior, a measure of spinal neuron sensitization. ERK signaling within the spinal cord is therefore involved in generating pain hypersensitivity. Because of its rapid activation, this effect probably involves regulation of neuronal excitability without changes in transcription.
Duke Scholars
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Related Subject Headings
- Temperature
- Signal Transduction
- Receptors, N-Methyl-D-Aspartate
- Rats, Sprague-Dawley
- Rats
- Protein Serine-Threonine Kinases
- Posterior Horn Cells
- Phosphorylation
- Pain Threshold
- Pain Measurement
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Temperature
- Signal Transduction
- Receptors, N-Methyl-D-Aspartate
- Rats, Sprague-Dawley
- Rats
- Protein Serine-Threonine Kinases
- Posterior Horn Cells
- Phosphorylation
- Pain Threshold
- Pain Measurement