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Metabolic control of oocyte apoptosis mediated by 14-3-3zeta-regulated dephosphorylation of caspase-2.

Publication ,  Journal Article
Nutt, LK; Buchakjian, MR; Gan, E; Darbandi, R; Yoon, S-Y; Wu, JQ; Miyamoto, YJ; Gibbons, JA; Andersen, JL; Freel, CD; Tang, W; He, C; Wang, Y ...
Published in: Developmental cell
June 2009

Xenopus oocyte death is partly controlled by the apoptotic initiator caspase-2 (C2). We reported previously that oocyte nutrient depletion activates C2 upstream of mitochondrial cytochrome c release. Conversely, nutrient-replete oocytes inhibit C2 via S135 phosphorylation catalyzed by calcium/calmodulin-dependent protein kinase II. We now show that C2 phosphorylated at S135 binds 14-3-3zeta, thus preventing C2 dephosphorylation. Moreover, we determined that S135 dephosphorylation is catalyzed by protein phosphatase-1 (PP1), which directly binds C2. Although C2 dephosphorylation is responsive to metabolism, neither PP1 activity nor binding is metabolically regulated. Rather, release of 14-3-3zeta from C2 is controlled by metabolism and allows for C2 dephosphorylation. Accordingly, a C2 mutant unable to bind 14-3-3zeta is highly susceptible to dephosphorylation. Although this mechanism was initially established in Xenopus, we now demonstrate similar control of murine C2 by phosphorylation and 14-3-3 binding in mouse eggs. These findings provide an unexpected evolutionary link between 14-3-3 and metabolism in oocyte death.

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Published In

Developmental cell

DOI

EISSN

1878-1551

ISSN

1534-5807

Publication Date

June 2009

Volume

16

Issue

6

Start / End Page

856 / 866

Related Subject Headings

  • Xenopus
  • Protein Phosphatase 1
  • Protein Binding
  • Phosphorylation
  • Oocytes
  • Mice
  • Female
  • Enzyme Activation
  • Developmental Biology
  • Caspase 2
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Nutt, L. K., Buchakjian, M. R., Gan, E., Darbandi, R., Yoon, S.-Y., Wu, J. Q., … Kornbluth, S. (2009). Metabolic control of oocyte apoptosis mediated by 14-3-3zeta-regulated dephosphorylation of caspase-2. Developmental Cell, 16(6), 856–866. https://doi.org/10.1016/j.devcel.2009.04.005
Nutt, Leta K., Marisa R. Buchakjian, Eugene Gan, Rashid Darbandi, Sook-Young Yoon, Judy Q. Wu, Yuko J. Miyamoto, et al. “Metabolic control of oocyte apoptosis mediated by 14-3-3zeta-regulated dephosphorylation of caspase-2.Developmental Cell 16, no. 6 (June 2009): 856–66. https://doi.org/10.1016/j.devcel.2009.04.005.
Nutt LK, Buchakjian MR, Gan E, Darbandi R, Yoon S-Y, Wu JQ, et al. Metabolic control of oocyte apoptosis mediated by 14-3-3zeta-regulated dephosphorylation of caspase-2. Developmental cell. 2009 Jun;16(6):856–66.
Nutt, Leta K., et al. “Metabolic control of oocyte apoptosis mediated by 14-3-3zeta-regulated dephosphorylation of caspase-2.Developmental Cell, vol. 16, no. 6, June 2009, pp. 856–66. Epmc, doi:10.1016/j.devcel.2009.04.005.
Nutt LK, Buchakjian MR, Gan E, Darbandi R, Yoon S-Y, Wu JQ, Miyamoto YJ, Gibbons JA, Andersen JL, Freel CD, Tang W, He C, Kurokawa M, Wang Y, Margolis SS, Fissore RA, Kornbluth S. Metabolic control of oocyte apoptosis mediated by 14-3-3zeta-regulated dephosphorylation of caspase-2. Developmental cell. 2009 Jun;16(6):856–866.
Journal cover image

Published In

Developmental cell

DOI

EISSN

1878-1551

ISSN

1534-5807

Publication Date

June 2009

Volume

16

Issue

6

Start / End Page

856 / 866

Related Subject Headings

  • Xenopus
  • Protein Phosphatase 1
  • Protein Binding
  • Phosphorylation
  • Oocytes
  • Mice
  • Female
  • Enzyme Activation
  • Developmental Biology
  • Caspase 2