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Analysis of the cell cycle using Xenopus egg extracts.

Publication ,  Journal Article
Kornbluth, S; Yang, J; Powers, M
Published in: Current protocols in cell biology
January 2006

In this unit, Xenopus eggs are isolated from hormonally primed female frogs, and then the extract is treated with cyclohexamide so it remains in interphase of the cell cycle. In the presence of sperm chromatin and ATP, membrane vesicles in the extract fuse to assemble nuclei, making the extract suitable for studies of DNA replication and nuclear transport.

Duke Scholars

Published In

Current protocols in cell biology

DOI

EISSN

1934-2616

ISSN

1934-2500

Publication Date

January 2006

Volume

Chapter 11

Start / End Page

Unit / 11.11

Related Subject Headings

  • Xenopus laevis
  • Transport Vesicles
  • Oocytes
  • Molecular Biology
  • Interphase
  • Female
  • DNA Replication
  • Cell Fractionation
  • Cell Extracts
  • Cell Cycle
 

Citation

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Kornbluth, S., Yang, J., & Powers, M. (2006). Analysis of the cell cycle using Xenopus egg extracts. Current Protocols in Cell Biology, Chapter 11, Unit-11.11. https://doi.org/10.1002/0471143030.cb1111s29
Kornbluth, Sally, Jing Yang, and Maureen Powers. “Analysis of the cell cycle using Xenopus egg extracts.Current Protocols in Cell Biology Chapter 11 (January 2006): Unit-11.11. https://doi.org/10.1002/0471143030.cb1111s29.
Kornbluth S, Yang J, Powers M. Analysis of the cell cycle using Xenopus egg extracts. Current protocols in cell biology. 2006 Jan;Chapter 11:Unit-11.11.
Kornbluth, Sally, et al. “Analysis of the cell cycle using Xenopus egg extracts.Current Protocols in Cell Biology, vol. Chapter 11, Jan. 2006, p. Unit-11.11. Epmc, doi:10.1002/0471143030.cb1111s29.
Kornbluth S, Yang J, Powers M. Analysis of the cell cycle using Xenopus egg extracts. Current protocols in cell biology. 2006 Jan;Chapter 11:Unit-11.11.

Published In

Current protocols in cell biology

DOI

EISSN

1934-2616

ISSN

1934-2500

Publication Date

January 2006

Volume

Chapter 11

Start / End Page

Unit / 11.11

Related Subject Headings

  • Xenopus laevis
  • Transport Vesicles
  • Oocytes
  • Molecular Biology
  • Interphase
  • Female
  • DNA Replication
  • Cell Fractionation
  • Cell Extracts
  • Cell Cycle