Analysis of the cell cycle using Xenopus egg extracts.
Publication
, Journal Article
Kornbluth, S; Yang, J; Powers, M
Published in: Current protocols in cell biology
January 2006
In this unit, Xenopus eggs are isolated from hormonally primed female frogs, and then the extract is treated with cyclohexamide so it remains in interphase of the cell cycle. In the presence of sperm chromatin and ATP, membrane vesicles in the extract fuse to assemble nuclei, making the extract suitable for studies of DNA replication and nuclear transport.
Duke Scholars
Published In
Current protocols in cell biology
DOI
EISSN
1934-2616
ISSN
1934-2500
Publication Date
January 2006
Volume
Chapter 11
Start / End Page
Unit / 11.11
Related Subject Headings
- Xenopus laevis
- Transport Vesicles
- Oocytes
- Molecular Biology
- Interphase
- Female
- DNA Replication
- Cell Fractionation
- Cell Extracts
- Cell Cycle
Citation
APA
Chicago
ICMJE
MLA
NLM
Kornbluth, S., Yang, J., & Powers, M. (2006). Analysis of the cell cycle using Xenopus egg extracts. Current Protocols in Cell Biology, Chapter 11, Unit-11.11. https://doi.org/10.1002/0471143030.cb1111s29
Kornbluth, Sally, Jing Yang, and Maureen Powers. “Analysis of the cell cycle using Xenopus egg extracts.” Current Protocols in Cell Biology Chapter 11 (January 2006): Unit-11.11. https://doi.org/10.1002/0471143030.cb1111s29.
Kornbluth S, Yang J, Powers M. Analysis of the cell cycle using Xenopus egg extracts. Current protocols in cell biology. 2006 Jan;Chapter 11:Unit-11.11.
Kornbluth, Sally, et al. “Analysis of the cell cycle using Xenopus egg extracts.” Current Protocols in Cell Biology, vol. Chapter 11, Jan. 2006, p. Unit-11.11. Epmc, doi:10.1002/0471143030.cb1111s29.
Kornbluth S, Yang J, Powers M. Analysis of the cell cycle using Xenopus egg extracts. Current protocols in cell biology. 2006 Jan;Chapter 11:Unit-11.11.
Published In
Current protocols in cell biology
DOI
EISSN
1934-2616
ISSN
1934-2500
Publication Date
January 2006
Volume
Chapter 11
Start / End Page
Unit / 11.11
Related Subject Headings
- Xenopus laevis
- Transport Vesicles
- Oocytes
- Molecular Biology
- Interphase
- Female
- DNA Replication
- Cell Fractionation
- Cell Extracts
- Cell Cycle