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Effect of microRNA modulation on bioartificial muscle function.

Publication ,  Journal Article
Rhim, C; Cheng, CS; Kraus, WE; Truskey, GA
Published in: Tissue Eng Part A
December 2010

Cellular therapies have recently employed the use of small RNA molecules, particularly microRNAs (miRNAs), to regulate various cellular processes that may be altered in disease states. In this study, we examined the effect of transient muscle-specific miRNA inhibition on the function of three-dimensional skeletal muscle cultures, or bioartificial muscles (BAMs). Skeletal myoblast differentiation in vitro is enhanced by inhibiting a proliferation-promoting miRNA (miR-133) expressed in muscle tissues. As assessed by functional force measurements in response to electrical stimulation at frequencies ranging from 0 to 20 Hz, peak forces exhibited by BAMs with miR-133 inhibition (anti-miR-133) were on average 20% higher than the corresponding negative control, although dynamic responses to electrical stimulation in miRNA-transfected BAMs and negative controls were similar to nontransfected controls. Immunostaining for alpha-actinin and myosin also showed more distinct striations and myofiber organization in anti-miR-133 BAMs, and fiber diameters were significantly larger in these BAMs over both the nontransfected and negative controls. Compared to the negative control, anti-miR-133 BAMs exhibited more intense nuclear staining for Mef2, a key myogenic differentiation marker. To our knowledge, this study is the first to demonstrate that miRNA mediation has functional effects on tissue-engineered constructs.

Duke Scholars

Published In

Tissue Eng Part A

DOI

EISSN

1937-335X

Publication Date

December 2010

Volume

16

Issue

12

Start / End Page

3589 / 3597

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Myosins
  • Myoblasts, Skeletal
  • Muscle, Skeletal
  • MicroRNAs
  • Mice
  • Cell Proliferation
  • Cell Line
  • Cell Differentiation
  • Biomedical Engineering
 

Citation

APA
Chicago
ICMJE
MLA
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Rhim, C., Cheng, C. S., Kraus, W. E., & Truskey, G. A. (2010). Effect of microRNA modulation on bioartificial muscle function. Tissue Eng Part A, 16(12), 3589–3597. https://doi.org/10.1089/ten.TEA.2009.0601
Rhim, Caroline, Cindy S. Cheng, William E. Kraus, and George A. Truskey. “Effect of microRNA modulation on bioartificial muscle function.Tissue Eng Part A 16, no. 12 (December 2010): 3589–97. https://doi.org/10.1089/ten.TEA.2009.0601.
Rhim C, Cheng CS, Kraus WE, Truskey GA. Effect of microRNA modulation on bioartificial muscle function. Tissue Eng Part A. 2010 Dec;16(12):3589–97.
Rhim, Caroline, et al. “Effect of microRNA modulation on bioartificial muscle function.Tissue Eng Part A, vol. 16, no. 12, Dec. 2010, pp. 3589–97. Pubmed, doi:10.1089/ten.TEA.2009.0601.
Rhim C, Cheng CS, Kraus WE, Truskey GA. Effect of microRNA modulation on bioartificial muscle function. Tissue Eng Part A. 2010 Dec;16(12):3589–3597.

Published In

Tissue Eng Part A

DOI

EISSN

1937-335X

Publication Date

December 2010

Volume

16

Issue

12

Start / End Page

3589 / 3597

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Myosins
  • Myoblasts, Skeletal
  • Muscle, Skeletal
  • MicroRNAs
  • Mice
  • Cell Proliferation
  • Cell Line
  • Cell Differentiation
  • Biomedical Engineering