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Abnormal gene expression profiles in unaffected parents of patients with hereditary-type retinoblastoma.

Publication ,  Journal Article
Chuang, EY; Chen, X; Tsai, M-H; Yan, H; Li, C-Y; Mitchell, JB; Nagasawa, H; Wilson, PF; Peng, Y; Fitzek, MM; Bedford, JS; Little, JB
Published in: Cancer Res
April 1, 2006

The hereditary form of retinoblastoma (Rb) is associated with a germ line mutation in one RB allele and is characterized by the occurrence of multiple, bilateral Rb tumors and a predisposition to the development of second cancers. In an earlier study, we observed an unexpected hypersensitivity to ionizing radiation in skin fibroblasts derived from unaffected parents of children with hereditary Rb. In at least four of these five families, there was no family history of Rb, indicating a new germ line mutation. We hypothesize that the increased parental cell sensitivity to radiation may reflect the presence of an as yet unrecognized genetic abnormality occurring in one or both parents of children with Rb. In the present study, we use DNA microarray technology to determine whether differences in gene expression profiles occurred in the unaffected parents of patients with hereditary Rb relative to normal individuals. Microarray analyses were validated by quantitative reverse transcription-PCR measurements. A distinct difference was observed in the patterns of gene expression between unaffected Rb parents and normal controls. By use of the prediction analysis for microarrays and principal component analysis methodologies, significant differences between the two groups were identified when as few as nine genes were analyzed. Further study of this phenomenon may offer a new insight into the genetic mechanisms of Rb and perhaps more broadly in cancer biology.

Duke Scholars

Published In

Cancer Res

DOI

ISSN

0008-5472

Publication Date

April 1, 2006

Volume

66

Issue

7

Start / End Page

3428 / 3433

Location

United States

Related Subject Headings

  • Skin
  • Reverse Transcriptase Polymerase Chain Reaction
  • Retinoblastoma
  • Retinal Neoplasms
  • Radiation Tolerance
  • RNA
  • Parents
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Humans
 

Citation

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Chuang, E. Y., Chen, X., Tsai, M.-H., Yan, H., Li, C.-Y., Mitchell, J. B., … Little, J. B. (2006). Abnormal gene expression profiles in unaffected parents of patients with hereditary-type retinoblastoma. Cancer Res, 66(7), 3428–3433. https://doi.org/10.1158/0008-5472.CAN-05-2847
Chuang, Eric Y., Xi Chen, Mong-Hsun Tsai, Hailing Yan, Chuan-Yuan Li, James B. Mitchell, Hatsumi Nagasawa, et al. “Abnormal gene expression profiles in unaffected parents of patients with hereditary-type retinoblastoma.Cancer Res 66, no. 7 (April 1, 2006): 3428–33. https://doi.org/10.1158/0008-5472.CAN-05-2847.
Chuang EY, Chen X, Tsai M-H, Yan H, Li C-Y, Mitchell JB, et al. Abnormal gene expression profiles in unaffected parents of patients with hereditary-type retinoblastoma. Cancer Res. 2006 Apr 1;66(7):3428–33.
Chuang, Eric Y., et al. “Abnormal gene expression profiles in unaffected parents of patients with hereditary-type retinoblastoma.Cancer Res, vol. 66, no. 7, Apr. 2006, pp. 3428–33. Pubmed, doi:10.1158/0008-5472.CAN-05-2847.
Chuang EY, Chen X, Tsai M-H, Yan H, Li C-Y, Mitchell JB, Nagasawa H, Wilson PF, Peng Y, Fitzek MM, Bedford JS, Little JB. Abnormal gene expression profiles in unaffected parents of patients with hereditary-type retinoblastoma. Cancer Res. 2006 Apr 1;66(7):3428–3433.

Published In

Cancer Res

DOI

ISSN

0008-5472

Publication Date

April 1, 2006

Volume

66

Issue

7

Start / End Page

3428 / 3433

Location

United States

Related Subject Headings

  • Skin
  • Reverse Transcriptase Polymerase Chain Reaction
  • Retinoblastoma
  • Retinal Neoplasms
  • Radiation Tolerance
  • RNA
  • Parents
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Humans