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Optical measurement of transmembrane potential in intact cardiac tissues using fluorescent resonance energy transfer

Publication ,  Journal Article
LoSicco, T; Malkin, RA
Published in: Computers in Cardiology
December 1, 1998

Fluorescent Resonance Energy Transfer [FRET] is the transfer of excitation energy from a donor fluorescent molecule to an acceptor fluorescent molecule. FRET has been developed to achieve fast, ratiometric, transmembrane potential sensitive fluorescence changes in single cells. The mechanism relies on the rapid redistribution of Hydrophobic fluorescent onions. These onions redistribute from one face of the plasma membrane to the other according to the Nernst equation. A second fluorophore is used to label an exatracelluar surface and undergoes energy transfer with the membrane bound sensor. During membrane depolarization the FRET is disrupted. This large change in fluorescence allows for imaging of the transmembrane potential. It is the objective of this work to adapt the FRET measurement system for eventual use in intact cardiac tissues. Currently, studies are underway for the individual verification of the functionality of each dye placed in the FRET system. The fluorescent anion experiments have shown the expected decrease in fluorescent intensity with a decrease in transmembrane potential.

Duke Scholars

Published In

Computers in Cardiology

ISSN

0276-6574

Publication Date

December 1, 1998

Volume

0

Issue

0

Start / End Page

573 / 576

Related Subject Headings

  • Cardiovascular System & Hematology
 

Citation

APA
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ICMJE
MLA
NLM
LoSicco, T., & Malkin, R. A. (1998). Optical measurement of transmembrane potential in intact cardiac tissues using fluorescent resonance energy transfer. Computers in Cardiology, 0(0), 573–576.
LoSicco, T., and R. A. Malkin. “Optical measurement of transmembrane potential in intact cardiac tissues using fluorescent resonance energy transfer.” Computers in Cardiology 0, no. 0 (December 1, 1998): 573–76.
LoSicco, T., and R. A. Malkin. “Optical measurement of transmembrane potential in intact cardiac tissues using fluorescent resonance energy transfer.” Computers in Cardiology, vol. 0, no. 0, Dec. 1998, pp. 573–76.

Published In

Computers in Cardiology

ISSN

0276-6574

Publication Date

December 1, 1998

Volume

0

Issue

0

Start / End Page

573 / 576

Related Subject Headings

  • Cardiovascular System & Hematology