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A method to correct pulling geometry induced errors in single molecule force spectroscopy measurements

Publication ,  Journal Article
Rivera, M; Ke, C; Marszalek, PE; Cole, DG; Clark, RL
Published in: 2007 Proceedings of the ASME International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, DETC2007
June 13, 2008

In AFM-based single molecule force spectroscopy, it is assumed that the pulling angle is negligible and that the force applied to the molecule is equivalent to the force measured by the instrument. Although this assumption may hold for flexible, compact molecules, studies have shown that it may not be appropriate for fairly rigid molecules, where measured forces can be a fraction of the actual values experienced by the molecule. Because the pulling geometry can substantially influence the values measured by the AFM, we investigate a method to minimize the pulling angle prior to conducting a pulling experiment. The method presented herein uses small circular movements to locate the molecule's substrate attachment site and reposition the cantilever. By using data gathered from a previous study, we were able to repeatedly align a molecule's attachment sites via simulation of the program, thereby demonstrating the effectiveness of the alignment method. Copyright © 2007 by ASME.

Duke Scholars

Published In

2007 Proceedings of the ASME International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, DETC2007

DOI

Publication Date

June 13, 2008

Volume

3 PART B

Start / End Page

801 / 807
 

Citation

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Rivera, M., Ke, C., Marszalek, P. E., Cole, D. G., & Clark, R. L. (2008). A method to correct pulling geometry induced errors in single molecule force spectroscopy measurements. 2007 Proceedings of the ASME International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, DETC2007, 3 PART B, 801–807. https://doi.org/10.1115/DETC2007-35430
Rivera, M., C. Ke, P. E. Marszalek, D. G. Cole, and R. L. Clark. “A method to correct pulling geometry induced errors in single molecule force spectroscopy measurements.” 2007 Proceedings of the ASME International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, DETC2007 3 PART B (June 13, 2008): 801–7. https://doi.org/10.1115/DETC2007-35430.
Rivera M, Ke C, Marszalek PE, Cole DG, Clark RL. A method to correct pulling geometry induced errors in single molecule force spectroscopy measurements. 2007 Proceedings of the ASME International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, DETC2007. 2008 Jun 13;3 PART B:801–7.
Rivera, M., et al. “A method to correct pulling geometry induced errors in single molecule force spectroscopy measurements.” 2007 Proceedings of the ASME International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, DETC2007, vol. 3 PART B, June 2008, pp. 801–07. Scopus, doi:10.1115/DETC2007-35430.
Rivera M, Ke C, Marszalek PE, Cole DG, Clark RL. A method to correct pulling geometry induced errors in single molecule force spectroscopy measurements. 2007 Proceedings of the ASME International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, DETC2007. 2008 Jun 13;3 PART B:801–807.

Published In

2007 Proceedings of the ASME International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, DETC2007

DOI

Publication Date

June 13, 2008

Volume

3 PART B

Start / End Page

801 / 807