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Biochemical and radiohistochemical analyses of alpha-2 adrenergic receptors in the kindling model of epilepsy.

Publication ,  Journal Article
Chen, LS; Weingart, JB; McNamara, JO
Published in: J Pharmacol Exp Ther
June 1990

Biochemical and radiohistochemical methods were used to study alterations in alpha-2 adrenergic receptors in the kindling model of epilepsy. The radioligands used for antagonist and agonist binding were [3H]idazoxan and [3H]p-aminoclonidine, respectively. Radioligand binding to membranes isolated from eight brain regions disclosed a small (14%) but statistically significant reduction in [3H]idazoxan binding in a single area, amygdala-pyriform-entorhinal cortex. This was confirmed by a larger (25%) reduction (P less than .02) in agonist binding in the same region. These reductions were present in animals sacrificed 1 day, but not 2 weeks, after the last kindled seizure. Quantitative radiohistochemical studies identified a bilaterally symmetric reduction of approximately 20% of [3H]p-aminoclonidine binding in the pyriform cortex and the central, medial and lateral amygdaloid nuclei. No significant differences were detected in nine other structures. We propose that the reduction in alpha-2 receptor binding contributes in part to the reduced sensitivity of pyriform cortex neurons of kindled animals to the inhibitory effects of alpha 2 agonists. The reduced alpha-2-receptor binding may also underlie a transient attenuation of alpha-2 receptor-mediated neuro-transmission and thereby constitute one mechanism contributing to kindling development.

Duke Scholars

Published In

J Pharmacol Exp Ther

ISSN

0022-3565

Publication Date

June 1990

Volume

253

Issue

3

Start / End Page

1272 / 1277

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, alpha
  • Rats, Inbred Strains
  • Rats
  • Pharmacology & Pharmacy
  • Models, Neurological
  • Male
  • Kindling, Neurologic
  • Idazoxan
  • Epilepsy
  • Dioxanes
 

Citation

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MLA
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Chen, L. S., Weingart, J. B., & McNamara, J. O. (1990). Biochemical and radiohistochemical analyses of alpha-2 adrenergic receptors in the kindling model of epilepsy. J Pharmacol Exp Ther, 253(3), 1272–1277.
Chen, L. S., J. B. Weingart, and J. O. McNamara. “Biochemical and radiohistochemical analyses of alpha-2 adrenergic receptors in the kindling model of epilepsy.J Pharmacol Exp Ther 253, no. 3 (June 1990): 1272–77.
Chen LS, Weingart JB, McNamara JO. Biochemical and radiohistochemical analyses of alpha-2 adrenergic receptors in the kindling model of epilepsy. J Pharmacol Exp Ther. 1990 Jun;253(3):1272–7.
Chen, L. S., et al. “Biochemical and radiohistochemical analyses of alpha-2 adrenergic receptors in the kindling model of epilepsy.J Pharmacol Exp Ther, vol. 253, no. 3, June 1990, pp. 1272–77.
Chen LS, Weingart JB, McNamara JO. Biochemical and radiohistochemical analyses of alpha-2 adrenergic receptors in the kindling model of epilepsy. J Pharmacol Exp Ther. 1990 Jun;253(3):1272–1277.

Published In

J Pharmacol Exp Ther

ISSN

0022-3565

Publication Date

June 1990

Volume

253

Issue

3

Start / End Page

1272 / 1277

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, alpha
  • Rats, Inbred Strains
  • Rats
  • Pharmacology & Pharmacy
  • Models, Neurological
  • Male
  • Kindling, Neurologic
  • Idazoxan
  • Epilepsy
  • Dioxanes