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Gene expression and cell growth are modified by silencing SUMO2 and SUMO3 expression.

Publication ,  Journal Article
Yang, W; Paschen, W
Published in: Biochem Biophys Res Commun
April 24, 2009

Small ubiquitin-like modifier (SUMO) is a group of proteins binding to lysine residues of target proteins and thereby modifying their stability, activity and subcellular localization. Here we report that blocking SUMO2 and SUMO3 conjugation by silencing their expression markedly modifies gene expression. A microRNA-based RNAi system was used to specifically silence SUMO2 and SUMO3 expression simultaneously and stably transfected neuroblastoma B35 cells expressing dual SUMO2/3 microRNA were created. In cells stably expressing SUMO2/3 microRNA, mRNA levels of 105 and 58 known genes were significantly up- and down-regulated, respectively. About 20% of differentially regulated genes were associated with pathways involved in cell growth and differentiation. Cell division was significantly suppressed in SUMO2/3 miRNA expressing cells. Elucidating what effect the silencing of SUMO2/3 expression has on gene expression will help to identify the impact of SUMO2/3 conjugation on the various cellular pathways.

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Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

April 24, 2009

Volume

382

Issue

1

Start / End Page

215 / 218

Location

United States

Related Subject Headings

  • Small Ubiquitin-Related Modifier Proteins
  • Rats
  • RNA, Small Interfering
  • Gene Silencing
  • Gene Expression Regulation
  • Cell Proliferation
  • Cell Line, Tumor
  • Biochemistry & Molecular Biology
  • Animals
  • 3404 Medicinal and biomolecular chemistry
 

Citation

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Yang, W., & Paschen, W. (2009). Gene expression and cell growth are modified by silencing SUMO2 and SUMO3 expression. Biochem Biophys Res Commun, 382(1), 215–218. https://doi.org/10.1016/j.bbrc.2009.03.013
Yang, Wei, and Wulf Paschen. “Gene expression and cell growth are modified by silencing SUMO2 and SUMO3 expression.Biochem Biophys Res Commun 382, no. 1 (April 24, 2009): 215–18. https://doi.org/10.1016/j.bbrc.2009.03.013.
Yang W, Paschen W. Gene expression and cell growth are modified by silencing SUMO2 and SUMO3 expression. Biochem Biophys Res Commun. 2009 Apr 24;382(1):215–8.
Yang, Wei, and Wulf Paschen. “Gene expression and cell growth are modified by silencing SUMO2 and SUMO3 expression.Biochem Biophys Res Commun, vol. 382, no. 1, Apr. 2009, pp. 215–18. Pubmed, doi:10.1016/j.bbrc.2009.03.013.
Yang W, Paschen W. Gene expression and cell growth are modified by silencing SUMO2 and SUMO3 expression. Biochem Biophys Res Commun. 2009 Apr 24;382(1):215–218.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

April 24, 2009

Volume

382

Issue

1

Start / End Page

215 / 218

Location

United States

Related Subject Headings

  • Small Ubiquitin-Related Modifier Proteins
  • Rats
  • RNA, Small Interfering
  • Gene Silencing
  • Gene Expression Regulation
  • Cell Proliferation
  • Cell Line, Tumor
  • Biochemistry & Molecular Biology
  • Animals
  • 3404 Medicinal and biomolecular chemistry