Scholars@Duke

• Background
• 

  Education, Training, & Certifications

  • Ph.D., Universitat Rostock (Germany) 2005
  • M.S., Chinese Academy of Sciences (China) 2002
• 

  Duke Appointment History

  • Assistant Professor in Anesthesiology, Anesthesiology, Clinical Science Departments 2009 - 2017
• Research
• 

  Selected Grants

  • Head-mounted Photoacoustic Imaging of Deep-brain Neural Activities in Freely Behaving Animals awarded by National Institutes of Health 2020 - 2023
  • Mast cell activation as a determinant of neurologic injury after cardiac arrest awarded by National Institutes of Health 2020 - 2022
  • Enhanced neutrophil SUMOylation as a key to anti-inflammatory effects of hypothermia awarded by  American Heart Association 2019 - 2021
  • The Unfolded Protein Response and Neuroprotection in Stroke awarded by National Institutes of Health 2016 - 2021
  • Cognitive Effects of Body Temperature During Hypothermic Circulatory Arrest awarded by National Institutes of Health 2016 - 2021
  • Effect of Aging on Brain Ischemia/Stroke Outcome; Pathways, Mechanisms, and Rescue awarded by National Institutes of Health 2016 - 2020
  • Role of SUMO Conjugation in Ischemia: Significance, Mechanisms and Pathways awarded by National Institutes of Health 2012 - 2017
  • Restoration of Endoplasmic Reticulum Function in Experimental Stroke awarded by National Institutes of Health 2012 - 2015
  • SUMO Conjugation and Deep Hypothermia-Induced Organ Protection awarded by National Institutes of Health 2010 - 2014
  • Shutdown of Translation and Ischemia/Stroke-Induced Cell Death awarded by National Institutes of Health 2008 - 2010
  • Conditional Gene Silencing in Ischemia/Stroke Research awarded by National Institutes of Health 2008 - 2010
• Publications & Artistic Works
• 

  Selected Publications

  • Academic Articles

    • Jiang, Maorong, Ran Li, Jingjun Lyu, Xuan Li, Wei Wang, Zhuoran Wang, Huaxin Sheng, Weiguo Zhang, Jörn Karhausen, and Wei Yang. “MCC950, a selective NLPR3 inflammasome inhibitor, improves neurologic function and survival after cardiac arrest and resuscitation.” J Neuroinflammation 17, no. 1 (August 31, 2020): 256. https://doi.org/10.1186/s12974-020-01933-y.
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    • Wang, Ya-Chao, Francesca Galeffi, Wei Wang, Xuan Li, Liping Lu, Huaxin Sheng, Ulrike Hoffmann, Dennis A. Turner, and Wei Yang. “Chemogenetics-mediated acute inhibition of excitatory neuronal activity improves stroke outcome.” Exp Neurol 326 (April 2020): 113206. https://doi.org/10.1016/j.expneurol.2020.113206.
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    • Bernstock, Joshua D., Daniel G. Ye, Dagoberto Estevez, Gustavo Chagoya, Ya-Chao Wang, Florian Gessler, John M. Hallenbeck, and Wei Yang. “The Role of SUMOylation and Ubiquitination in Brain Ischaemia: Critical Concepts and Clinical Implications.” Curr Issues Mol Biol 35 (2020): 127–44. https://doi.org/10.21775/cimb.035.127.
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    • Ma, Shuang, Dongmei Chu, Litao Li, Jennifer A. Creed, Yu-Mi Ryang, Huaxin Sheng, Wei Yang, David S. Warner, Dennis A. Turner, and Ulrike Hoffmann. “Argon Inhalation for 24 Hours After Onset of Permanent Focal Cerebral Ischemia in Rats Provides Neuroprotection and Improves Neurologic Outcome.” Crit Care Med 47, no. 8 (August 2019): e693–99. https://doi.org/10.1097/CCM.0000000000003809.
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    • Wang, Zhuoran, and Wei Yang. “Impaired capacity to restore proteostasis in the aged brain after ischemia: Implications for translational brain ischemia research.” Neurochem Int 127 (July 2019): 87–93. https://doi.org/10.1016/j.neuint.2018.12.018.
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    • Lan, Bangxin, Wei Liu, Ya-Chao Wang, Junhui Shi, Yang Li, Song Xu, Huaxin Sheng, et al. “High-speed widefield photoacoustic microscopy of small-animal hemodynamics.” Biomed Opt Express 9, no. 10 (October 1, 2018): 4689–4701. https://doi.org/10.1364/BOE.9.004689.
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    • Shen, Yuntian, Baihui Yan, Qiang Zhao, Zhuoran Wang, Jiangbo Wu, Jiafa Ren, Wei Wang, et al. “Aging Is Associated With Impaired Activation of Protein Homeostasis-Related Pathways After Cardiac Arrest in Mice.” J Am Heart Assoc 7, no. 17 (September 4, 2018): e009634. https://doi.org/10.1161/JAHA.118.009634.
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    • Karhausen, Jörn, Joshua D. Bernstock, Kory R. Johnson, Huaxin Sheng, Qing Ma, Yuntian Shen, Wei Yang, John M. Hallenbeck, and Wulf Paschen. “Ubc9 overexpression and SUMO1 deficiency blunt inflammation after intestinal ischemia/reperfusion.” Lab Invest 98, no. 6 (June 2018): 799–813. https://doi.org/10.1038/s41374-018-0035-6.
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    • Bernstock, Joshua D., Daniel Ye, Jayden A. Smith, Yang-Ja Lee, Florian A. Gessler, Adam Yasgar, Jennifer Kouznetsova, et al. “Quantitative high-throughput screening identifies cytoprotective molecules that enhance SUMO conjugation via the inhibition of SUMO-specific protease (SENP)2.” Faseb J 32, no. 3 (March 2018): 1677–91. https://doi.org/10.1096/fj.201700711R.
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    • Liu, Huaqin, Zhui Yu, Ying Li, Bin Xu, Baihui Yan, Wulf Paschen, David S. Warner, Wei Yang, and Huaxin Sheng. “Novel Modification of Potassium Chloride Induced Cardiac Arrest Model for Aged Mice.” Aging Dis 9, no. 1 (February 2018): 31–39. https://doi.org/10.14336/AD.2017.0221.
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    • Bernstock, Joshua D., Wei Yang, Daniel G. Ye, Yuntian Shen, Stefano Pluchino, Yang-Ja Lee, John M. Hallenbeck, and Wulf Paschen. “SUMOylation in brain ischemia: Patterns, targets, and translational implications.” J Cereb Blood Flow Metab 38, no. 1 (January 2018): 5–16. https://doi.org/10.1177/0271678X17742260.
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    • Yang, Wei, and Wulf Paschen. “Is age a key factor contributing to the disparity between success of neuroprotective strategies in young animals and limited success in elderly stroke patients? Focus on protein homeostasis.” J Cereb Blood Flow Metab 37, no. 10 (October 2017): 3318–24. https://doi.org/10.1177/0271678X17723783.
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    • Bernstock, Joshua D., Daniel Ye, Florian A. Gessler, Yang-Ja Lee, Luca Peruzzotti-Jametti, Peter Baumgarten, Kory R. Johnson, et al. “Topotecan is a potent inhibitor of SUMOylation in glioblastoma multiforme and alters both cellular replication and metabolic programming.” Sci Rep 7, no. 1 (August 7, 2017): 7425. https://doi.org/10.1038/s41598-017-07631-9.
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    • Jiang, Meng, Shu Yu, Zhui Yu, Huaxin Sheng, Ying Li, Shuai Liu, David S. Warner, Wulf Paschen, and Wei Yang. “XBP1 (X-Box-Binding Protein-1)-Dependent O-GlcNAcylation Is Neuroprotective in Ischemic Stroke in Young Mice and Its Impairment in Aged Mice Is Rescued by Thiamet-G.” Stroke 48, no. 6 (June 2017): 1646–54. https://doi.org/10.1161/STROKEAHA.117.016579.
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    • Yu, Zhui, Huaxin Sheng, Shuai Liu, Shengli Zhao, Christopher C. Glembotski, David S. Warner, Wulf Paschen, and Wei Yang. “Activation of the ATF6 branch of the unfolded protein response in neurons improves stroke outcome.” J Cereb Blood Flow Metab 37, no. 3 (March 2017): 1069–79. https://doi.org/10.1177/0271678X16650218.
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    • Zhang, Lin, Xiaozhi Liu, Huaxin Sheng, Shuai Liu, Ying Li, Julia Q. Zhao, David S. Warner, Wulf Paschen, and Wei Yang. “Neuron-specific SUMO knockdown suppresses global gene expression response and worsens functional outcome after transient forebrain ischemia in mice.” Neuroscience 343 (February 20, 2017): 190–212. https://doi.org/10.1016/j.neuroscience.2016.11.036.
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    • Yang, Wei, and Wulf Paschen. “Unfolded protein response in brain ischemia: A timely update.” J Cereb Blood Flow Metab 36, no. 12 (December 2016): 2044–50. https://doi.org/10.1177/0271678X16674488.
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    • Yang, Wei, Huaxin Sheng, and Haichen Wang. “Targeting the SUMO pathway for neuroprotection in brain ischaemia.” Stroke Vasc Neurol 1, no. 3 (September 2016): 101–7. https://doi.org/10.1136/svn-2016-000031.
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    • Liu, Shuai, Huaxin Sheng, Zhui Yu, Wulf Paschen, and Wei Yang. “O-linked β-N-acetylglucosamine modification of proteins is activated in post-ischemic brains of young but not aged mice: Implications for impaired functional recovery from ischemic stress.” J Cereb Blood Flow Metab 36, no. 2 (February 2016): 393–98. https://doi.org/10.1177/0271678X15608393.
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    • Yang, Wei, and Wulf Paschen. “SUMO proteomics to decipher the SUMO-modified proteome regulated by various diseases.” Proteomics 15, no. 5–6 (March 2015): 1181–91. https://doi.org/10.1002/pmic.201400298.
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    • Wang, Liangli, Carolien Wansleeben, Shengli Zhao, Pei Miao, Wulf Paschen, and Wei Yang. “SUMO2 is essential while SUMO3 is dispensable for mouse embryonic development.” Embo Rep 15, no. 8 (August 2014): 878–85. https://doi.org/10.15252/embr.201438534.
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    • Wang, Liangli, Ramona M. Rodriguiz, William C. Wetsel, Huaxin Sheng, Shengli Zhao, Xiaozhi Liu, Wulf Paschen, and Wei Yang. “Neuron-specific Sumo1-3 knockdown in mice impairs episodic and fear memories.” J Psychiatry Neurosci 39, no. 4 (July 2014): 259–66. https://doi.org/10.1503/jpn.130148.
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    • Yang, Wei, Huaxin Sheng, J Will Thompson, Shengli Zhao, Liangli Wang, Pei Miao, Xiaozhi Liu, M Arthur Moseley, and Wulf Paschen. “Small ubiquitin-like modifier 3-modified proteome regulated by brain ischemia in novel small ubiquitin-like modifier transgenic mice: putative protective proteins/pathways.” Stroke 45, no. 4 (April 2014): 1115–22. https://doi.org/10.1161/STROKEAHA.113.004315.
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    • Iwabuchi, Masahiro, Huaxin Sheng, J Will Thompson, Liangli Wang, Laura G. Dubois, David Gooden, Marthur Moseley, Wulf Paschen, and Wei Yang. “Characterization of the ubiquitin-modified proteome regulated by transient forebrain ischemia.” J Cereb Blood Flow Metab 34, no. 3 (March 2014): 425–32. https://doi.org/10.1038/jcbfm.2013.210.
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    • Brietzke, Andreas, Tom Goldammer, Henrike Rebl, Tomáš Korytář, Bernd Köllner, Wei Yang, Alexander Rebl, and Hans-Martin Seyfert. “Characterization of the interleukin 1 receptor-associated kinase 4 (IRAK4)-encoding gene in salmonid fish: the functional copy is rearranged in Oncorhynchus mykiss and that factor can impair TLR signaling in mammalian cells.” Fish Shellfish Immunol 36, no. 1 (January 2014): 206–14. https://doi.org/10.1016/j.fsi.2013.11.005.
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    • Yang, Wei, Liangli Wang, and Wulf Paschen. “Development of a high-throughput screening assay for inhibitors of small ubiquitin-like modifier proteases.” J Biomol Screen 18, no. 5 (June 2013): 621–28. https://doi.org/10.1177/1087057113479971.
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    • Wang, Z., R. Wang, H. Sheng, S. P. Sheng, W. Paschen, and W. Yang. “Transient ischemia induces massive nuclear accumulation of SUMO2/3-conjugated proteins in spinal cord neurons.” Spinal Cord 51, no. 2 (February 2013): 139–43. https://doi.org/10.1038/sc.2012.100.
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    • Yang, Wei, Liangli Wang, Gabriele Roehn, Robert D. Pearlstein, Francis Ali-Osman, Hongjie Pan, Roland Goldbrunner, Matthew Krantz, Christoph Harms, and Wulf Paschen. “Small ubiquitin-like modifier 1-3 conjugation [corrected] is activated in human astrocytic brain tumors and is required for glioblastoma cell survival.” Cancer Sci 104, no. 1 (January 2013): 70–77. https://doi.org/10.1111/cas.12047.
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    • Wang, Liangli, Qing Ma, Wei Yang, G Burkhard Mackensen, and Wulf Paschen. “Moderate hypothermia induces marked increase in levels and nuclear accumulation of SUMO2/3-conjugated proteins in neurons.” J Neurochem 123, no. 3 (November 2012): 349–59. https://doi.org/10.1111/j.1471-4159.2012.07916.x.
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    • Yang, Wei, J Will Thompson, Zhengfeng Wang, Liangli Wang, Huaxin Sheng, Matthew W. Foster, M Arthur Moseley, and Wulf Paschen. “Analysis of oxygen/glucose-deprivation-induced changes in SUMO3 conjugation using SILAC-based quantitative proteomics.” J Proteome Res 11, no. 2 (February 3, 2012): 1108–17. https://doi.org/10.1021/pr200834f.
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    • Datwyler, Anna Lena, Gisela Lättig-Tünnemann, Wei Yang, Wulf Paschen, Sabrina Lin Lin Lee, Ulrich Dirnagl, Matthias Endres, and Christoph Harms. “SUMO2/3 conjugation is an endogenous neuroprotective mechanism.” J Cereb Blood Flow Metab 31, no. 11 (November 2011): 2152–59. https://doi.org/10.1038/jcbfm.2011.112.
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    • Wang, Zhengfeng, Wei Yang, Gavin W. Britz, Frederick W. Lombard, David S. Warner, and Huaxin Sheng. “Development of a simplified spinal cord ischemia model in mice.” J Neurosci Methods 189, no. 2 (June 15, 2010): 246–51. https://doi.org/10.1016/j.jneumeth.2010.04.003.
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    • Sheng, Huaxin, Wei Yang, Shiro Fukuda, Hubert M. Tse, Wulf Paschen, Kwame Johnson, Ines Batinic-Haberle, et al. “Long-term neuroprotection from a potent redox-modulating metalloporphyrin in the rat.” Free Radic Biol Med 47, no. 7 (October 1, 2009): 917–23. https://doi.org/10.1016/j.freeradbiomed.2009.05.039.
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    • Yang, Wei, and Wulf Paschen. “The endoplasmic reticulum and neurological diseases.” Exp Neurol 219, no. 2 (October 2009): 376–81. https://doi.org/10.1016/j.expneurol.2009.07.009.
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    • Günther, Juliane, Dirk Koczan, Wei Yang, Gerd Nürnberg, Dirk Repsilber, Hans-Joachim Schuberth, Zaneta Park, Nauman Maqbool, Adrian Molenaar, and Hans-Martin Seyfert. “Assessment of the immune capacity of mammary epithelial cells: comparison with mammary tissue after challenge with Escherichia coli.” Vet Res 40, no. 4 (July 2009): 31. https://doi.org/10.1051/vetres/2009014.
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    • Yang, Wei, Qing Ma, G Burkhard Mackensen, and Wulf Paschen. “Deep hypothermia markedly activates the small ubiquitin-like modifier conjugation pathway; implications for the fate of cells exposed to transient deep hypothermic cardiopulmonary bypass.” J Cereb Blood Flow Metab 29, no. 5 (May 2009): 886–90. https://doi.org/10.1038/jcbfm.2009.16.
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    • Yang, Wei, and Wulf Paschen. “Gene expression and cell growth are modified by silencing SUMO2 and SUMO3 expression.” Biochem Biophys Res Commun 382, no. 1 (April 24, 2009): 215–18. https://doi.org/10.1016/j.bbrc.2009.03.013.
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    • Yang, Wei, Huaxin Sheng, H Mayumi Homi, David S. Warner, and Wulf Paschen. “Cerebral ischemia/stroke and small ubiquitin-like modifier (SUMO) conjugation--a new target for therapeutic intervention?” J Neurochem 106, no. 3 (August 2008): 989–99. https://doi.org/10.1111/j.1471-4159.2008.05404.x.
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    • Yang, Wei, Huaxin Sheng, David S. Warner, and Wulf Paschen. “Transient focal cerebral ischemia induces a dramatic activation of small ubiquitin-like modifier conjugation.” J Cereb Blood Flow Metab 28, no. 5 (May 2008): 892–96. https://doi.org/10.1038/sj.jcbfm.9600601.
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    • Petzl, Wolfram, Holm Zerbe, Juliane Günther, Wei Yang, Hans-Martin Seyfert, Gerd Nürnberg, and Hans-Joachim Schuberth. “Escherichia coli, but not Staphylococcus aureus triggers an early increased expression of factors contributing to the innate immune defense in the udder of the cow.” Vet Res 39, no. 2 (March 2008): 18. https://doi.org/10.1051/vetres:2007057.
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    • Yang, Wei, Holm Zerbe, Wolfram Petzl, Ronald Marco Brunner, Juliane Günther, Christian Draing, Sonja von Aulock, Hans-Joachim Schuberth, and Hans-Martin Seyfert. “Bovine TLR2 and TLR4 properly transduce signals from Staphylococcus aureus and E. coli, but S. aureus fails to both activate NF-kappaB in mammary epithelial cells and to quickly induce TNFalpha and interleukin-8 (CXCL8) expression in the udder.” Mol Immunol 45, no. 5 (March 2008): 1385–97. https://doi.org/10.1016/j.molimm.2007.09.004.
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    • Yang, Wei, Huaxin Sheng, David S. Warner, and Wulf Paschen. “Transient global cerebral ischemia induces a massive increase in protein sumoylation.” J Cereb Blood Flow Metab 28, no. 2 (February 2008): 269–79. https://doi.org/10.1038/sj.jcbfm.9600523.
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    • Yang, Wei, and Wulf Paschen. “Conditional gene silencing in mammalian cells mediated by a stress-inducible promoter.” Biochem Biophys Res Commun 365, no. 3 (January 18, 2008): 521–27. https://doi.org/10.1016/j.bbrc.2007.11.011.
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    • Jaffrézic, Florence, Dirk-Jan de Koning, Paul J. Boettcher, Agnès Bonnet, Bart Buitenhuis, Rodrigue Closset, Sébastien Déjean, et al. “Analysis of the real EADGENE data set: comparison of methods and guidelines for data normalisation and selection of differentially expressed genes (open access publication).” Genet Sel Evol 39, no. 6 (November 2007): 633–50. https://doi.org/10.1186/1297-9686-39-6-633.
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    • Sørensen, Peter, Agnès Bonnet, Bart Buitenhuis, Rodrigue Closset, Sébastien Déjean, Céline Delmas, Mylène Duval, et al. “Analysis of the real EADGENE data set: multivariate approaches and post analysis (open access publication).” Genet Sel Evol 39, no. 6 (November 2007): 651–68. https://doi.org/10.1186/1297-9686-39-6-651.
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    • Bánhegyi, Gábor, Peter Baumeister, Angelo Benedetti, Dezheng Dong, Yong Fu, Amy S. Lee, Jianze Li, et al. “Endoplasmic reticulum stress.” Ann N Y Acad Sci 1113 (October 2007): 58–71. https://doi.org/10.1196/annals.1391.007.
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    • Vanselow, Jens, Wei Yang, Jens Herrmann, Holm Zerbe, Hans-Joachim Schuberth, Wolfram Petzl, Wolfgang Tomek, and Hans-Martin Seyfert. “DNA-remethylation around a STAT5-binding enhancer in the alphaS1-casein promoter is associated with abrupt shutdown of alphaS1-casein synthesis during acute mastitis.” J Mol Endocrinol 37, no. 3 (December 2006): 463–77. https://doi.org/10.1677/jme.1.02131.
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    • Yang, Wei, Adrian Molenaar, Bianka Kurts-Ebert, and Hans-Martin Seyfert. “NF-kappaB factors are essential, but not the switch, for pathogen-related induction of the bovine beta-defensin 5-encoding gene in mammary epithelial cells.” Mol Immunol 43, no. 3 (February 2006): 210–25. https://doi.org/10.1016/j.molimm.2005.02.003.
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  • Book Sections

    • Thompson, J. W., M. Jiang, and W. Yang. “Proteomic analysis of SUMOylation in the post-ischemic brain.” In Neuromethods, 127:207–24, 2017. https://doi.org/10.1007/978-1-4939-7119-0_13.
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