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Spectral-domain differential interference contrast microscopy.

Publication ,  Journal Article
Zhu, Y; Shaked, NT; Satterwhite, LL; Wax, A
Published in: Optics letters
February 2011

We present a fiber-optic low-coherence imaging technique, termed spectral-domain differential interference contrast microscopy (SD-DIC), for quantitative DIC imaging of both reflective surfaces and transparent biological specimens. SD-DIC combines the common-path nature of a Nomarski DIC interferometer with the high sensitivity of spectral-domain low-coherence interferometry to obtain high-resolution, quantitative measurements of optical pathlength gradients from a single point on the sample. Full-field imaging can be achieved by scanning the sample. A reflected-light SD-DIC system was demonstrated using a USAF resolution target as the phase object. Live cardiomyocytes were also imaged, achieving a resolution of 36 pm for pathlength gradient measurements. The dynamics of cardiomyocyte contraction were recorded with high sensitivity at selected sites on the cells.

Duke Scholars

Published In

Optics letters

DOI

EISSN

1539-4794

ISSN

0146-9592

Publication Date

February 2011

Volume

36

Issue

4

Start / End Page

430 / 432

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Optics
  • Myocytes, Cardiac
  • Microscopy, Interference
  • Light
  • Interferometry
  • Equipment Design
  • Diagnostic Imaging
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhu, Y., Shaked, N. T., Satterwhite, L. L., & Wax, A. (2011). Spectral-domain differential interference contrast microscopy. Optics Letters, 36(4), 430–432. https://doi.org/10.1364/ol.36.000430
Zhu, Yizheng, Natan T. Shaked, Lisa L. Satterwhite, and Adam Wax. “Spectral-domain differential interference contrast microscopy.Optics Letters 36, no. 4 (February 2011): 430–32. https://doi.org/10.1364/ol.36.000430.
Zhu Y, Shaked NT, Satterwhite LL, Wax A. Spectral-domain differential interference contrast microscopy. Optics letters. 2011 Feb;36(4):430–2.
Zhu, Yizheng, et al. “Spectral-domain differential interference contrast microscopy.Optics Letters, vol. 36, no. 4, Feb. 2011, pp. 430–32. Epmc, doi:10.1364/ol.36.000430.
Zhu Y, Shaked NT, Satterwhite LL, Wax A. Spectral-domain differential interference contrast microscopy. Optics letters. 2011 Feb;36(4):430–432.
Journal cover image

Published In

Optics letters

DOI

EISSN

1539-4794

ISSN

0146-9592

Publication Date

February 2011

Volume

36

Issue

4

Start / End Page

430 / 432

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Optics
  • Myocytes, Cardiac
  • Microscopy, Interference
  • Light
  • Interferometry
  • Equipment Design
  • Diagnostic Imaging
  • Animals